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A molecular marker method for rice leaf hydrophilic gene

A technology of molecular markers and hydrophilic genes, applied in the direction of plant genetic improvement, botany equipment and methods, biochemical equipment and methods, etc., can solve problems such as adverse effects on plants, improve breeding efficiency, speed up breeding process, and clearly position Effect

Active Publication Date: 2017-01-11
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Leaf phenotypes can be identified at the seedling stage, which is the simplest, fastest and most effective method to identify varieties, but most of the marker traits have adverse effects on the plants themselves

Method used

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  • A molecular marker method for rice leaf hydrophilic gene
  • A molecular marker method for rice leaf hydrophilic gene
  • A molecular marker method for rice leaf hydrophilic gene

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Material method

[0024] 1.1 Plant material

[0025] Zhenhui 714 is a hybrid mid-indica restorer line bred by the Zhenjiang Agricultural Science Research Institute in the hilly area of ​​Jiangsu. In 2007, a mutant with a hydrophilic leaf epidermis was found in the population breeding of Zhenhui 714, and it was named "wax crystal-sparse leaf 3 (wsl3)". The wsl3 mutant was crossed with the wild-type parent and the japonica rice variety Wuyujing 3, and the parents, F 1 and F 2 , to investigate target traits and obtain targeting groups.

[0026] 1.2 Determination of mutant traits and analysis of physiological traits

[0027] At the heading stage, select the leaves of mutant wsl3 plants and the leaves of the same parts of their wild-type parents to extract epidermal wax, and measure it with reference to the method of Zhou Xiaoyun et al. [Journal of Hunan Agricultural University (Natural Science Edition) Volume 33 No. 3] The waxy structure of leaf epidermis was observ...

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Abstract

The invention discloses a set of primer pairs. The set of primer pairs contains a primer pair c3-16 and a primer pair c3-22, wherein the nucleotide sequences of the c3-16 are shown as SEQ ID NO:1 and SEQ ID NO:2, and the nucleotide sequences of the c3-22 are shown as SEQ ID NO:3 and SEQ ID NO:4. The invention also discloses an application of the primer pairs in the aspect of molecular markers for hydrophilic genes of rice leaves. The invention also discloses a molecular marker method for hydrophilic genes of rice leaves. A hydrophilic gene of rice leaves provided by the invention is a new gene, in the process of hybrid rice breeding, the adoption of a molecular marker for assisted selection contributes to the accurate and rapid transduction of the hydrophilic gene of rice leaves, and then the hydrophilic gene of rice leaves, as a marker character, is applied to the improvement of rice varieties.

Description

technical field [0001] The invention belongs to the field of plant molecular breeding, and in particular relates to a molecular marker method for leaf hydrophilic genes caused by lack of rice epidermis wax. In the process of hybrid rice breeding, using the molecular markers for auxiliary selection to introduce rice leaf hydrophilic genes can be used as Marker traits accelerate improvement of rice varieties. Background technique [0002] Plant epidermal wax is a layer of hydrophobic barrier composed of various lipid compounds, which covers the surface of most organs of terrestrial plants and is an important structure on the surface of plants. Epidermis wax has physiological functions such as preventing non-stomatal water loss of plants, keeping plant surfaces clean and waterproof, resisting pests and diseases, preventing damage to plants from harmful light, reflecting visible light, affecting leaf and fruit coloring, and preventing fruit cracking. Quality also has an importa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11A01H1/02A01H1/04
CPCA01H1/02C12Q1/6895C12Q2600/13
Inventor 龚红兵曾生元李闯林添资景德道钱华飞余波周义文盛生兰
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS