Automatic histocyte separator

An automatic separator and tissue cell technology, applied in the field of cell separation, can solve the problems of increased pollution, unevenness, labor, etc., and achieve the effect of reducing the influence of human factors, mild operation, and aseptic operation

Active Publication Date: 2015-04-29
江苏赛拉纳生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The above methods all need to manually cut the tissue block into 1.0mm before cell separation 3 Tiny tissue pieces of the same size are laborious, time-consuming, uneven, and increase the chance of contamination

Method used

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Embodiment

[0030] like figure 1 As shown, the tissue cell automatic separator for separating tissue into single cells disclosed in the present invention includes a separation tube, and a motor is installed under the separation tube. The speed of the motor is adjustable and reversible. , speed, direction) to run. The outside of the separation tube is also provided with a protective heat preservation cover, which is equipped with a temperature probe, a thermoelectric cooling chip and a circulating fan, and the temperature probe, a thermoelectric cooling chip and a circulating fan are respectively connected with a microprocessor to realize protection. The temperature in the heat preservation cover is automatically controlled at 37°C, which is conducive to the development of digestive enzyme activity. The microprocessor is also connected to the motor to control the rotation of the motor, and the microprocessor is also connected to a touch screen for inputting operating parameters and displa...

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Abstract

The invention discloses an automatic histocyte separator which is used for separating tissues into single cells. The separator comprises a separation tube and a motor, wherein the separation tube comprises a tube wall and a tube core in the tube wall; the top part of the tube wall is blocked; a tube cover covers the bottom part of the tube wall; the tube core comprises a stator sleeve and a rotor in the sleeve; a rotor joint is arranged on the tube cover; the rotor is connected with the rotor joint; the motor is located outside the separation tube, and is used for providing rotary power for the rotor through the rotor joint; and the inside of the tube core is sequentially divided into an archimedes spiral pipe area, a cell separation area and a negative pressure rotary cutting area from top to bottom. An automatic system with a special tube core is utilized; a microprocessor is used for defining special application program to optimize operation regulations; a specimen can be conveniently treated; the cell separation and preparation processes are standardized and programmed; and the effects on human factors are reduced.

Description

technical field [0001] The invention relates to cell separation, in particular to an automatic tissue cell separator. Background technique [0002] Tissue cells are important materials for life science research such as cell biology, molecular biology, immunology, genetics, and oncology. For example, the prerequisite for flow cytometry (FCM) detection is that the sample is a single cell suspension However, most solid tissues are difficult to make high-quality single-cell suspension samples, which greatly limits the analysis and research of FCM on solid tissues, especially solid tumors. Tissue and cell separators are used to disperse body tissues such as lung, liver, tumors, etc. Chemical treatment of detergents, needle aspiration, etc. [0003] In order to conveniently separate single cell suspensions from tissues and prepare tissue blocks into homogenate in a gentle, safe, time-saving, and standard automated method, BD Company of the United States has launched the Medimach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCC12M3/08G01N1/28
Inventor 何向锋强福林沈康施文王健
Owner 江苏赛拉纳生物医药有限公司
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