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Almond mass spectrometric detection characteristic sequence group and detection kit

A characteristic sequence and mass spectrometry detection technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as false positive results of antibodies, shorten the detection time, meet the needs of a large number of screening and confirmation work, and meet the requirements of quantitative detection Effect

Inactive Publication Date: 2015-04-29
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current detection method for almonds is enzyme-linked immunoassay, which can easily lead to false positive results due to the specificity of the antibody

Method used

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  • Almond mass spectrometric detection characteristic sequence group and detection kit
  • Almond mass spectrometric detection characteristic sequence group and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Sample: The bread package is marked with a statement stating that it contains almond ingredients, and it is detected by mass spectrometry.

[0048] Take 2 g of bread matrix, add 10ml of extraction buffer, shake at 60°C for 3h, centrifuge at 14000g for 10min, take the supernatant, add 1M DTT solution 1μl, 37°C for 2h, then add 1M IAA 5μl at room temperature for 40min. After the reaction, directly add 2 μL trypsin (200 ng / μL) and hydrolyze with shaking at 37 °C for 1.5 hours.

[0049] After enzymolysis is completed, centrifuge at 20,000 g for 5 minutes, take the supernatant, and directly inject the sample for analysis.

[0050] Liquid chromatography conditions:

[0051] Column: Hypersil GOLD aQ column (100 x 2.1 mm, 1.9 μm); sample volume: 10 μL; phase A: 0.1% formic acid aqueous solution; phase B: 0.1% formic acid acetonitrile solution; flow rate: 300 μL / min; analytical gradient : 0 – 2 min 5% B, 2 – 12 min 5%–95% B, 12–17 min 95% B, 17–18 min, 95%–5% B, 18–23 min 5% B.

[0052]...

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Abstract

The invention relates to an almond mass spectrometric detection characteristic sequence group and a detection kit and belongs to the field of food detection. The invention discloses an almond mass spectrometric detection characteristic sequence group, a reagent for almond allergen detection and an almond allergen detection method. The almond mass spectrometric detection characteristic sequence group and the detection kit disclosed by the invention are high in sensitivity and high in accuracy, are suitable for detecting almond content in bread, ice creams and other almond-containing processed products and identifying authenticity of the almond and are convenient to popularize and apply in various detection mechanisms.

Description

Technical field [0001] The present invention provides a method for detecting almonds, which belongs to the field of food detection, and specifically relates to a technique for detecting characteristic sequence groups in almonds by using a mass spectrometry method. Background technique [0002] Food allergies are a health problem that has been paid more and more attention. Nuts are one of the eight major food allergens, involving 90% of food allergies. Tree nut allergens are stable to food processing and digestion. In some seeds, similar stable allergens have been identified, because of their unique taste, seeds and nuts are often used in the food industry. Since the 1990s, due to the diversification of food products, there have been more and more recipes related to nuts and seeds. Products that do not contain nuts or seeds may use equipment that has processed nuts and seeds, forming a potential risk of cross-contact. The exploration of allergens in currently hypothetical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 贺艳郑文杰张裕君赵璟源张霞陈其勇赵卫东许泓
Owner ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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