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Method for enriching and detecting low-concentration phenol effective components

A technology with active ingredients and low concentration, which is applied in the field of extraction and preparation of natural medicines. It can solve the problems of single detection method, adsorbent, and few applications, and achieve novel ideas in the enrichment process, good thermal stability, and remarkable enrichment effect. Effect

Active Publication Date: 2015-04-29
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many literature reports on the detection of phenolic components in chrysanthemum, but the detection method and the adsorbent used are relatively simple.
So far, the use of chitosan-dispersed multi-walled carbon nanotubes as an adsorbent in solid-phase microextraction of traditional Chinese medicine has been seldom used.
And this method has not yet been reported in the chrysanthemum extraction and enrichment links

Method used

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  • Method for enriching and detecting low-concentration phenol effective components
  • Method for enriching and detecting low-concentration phenol effective components
  • Method for enriching and detecting low-concentration phenol effective components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1. Investigate the enrichment effect of the multi-walled carbon nanotube dispersion liquid dispersed in chitosan under different additions

[0051] 1.1 Take 5 clean 500mL Erlenmeyer flasks with stoppers, numbered 1, 2, 3, 4, 5, add 40 μL of 50 μg / mL reference substance mixture, and add 100 μg / mL chitosan-multi-walled carbon nano Tube dispersion 50 μL and pH=7 water 19.91mL, No. 2, add 100 μg / mL chitosan-multi-walled carbon nanotube dispersion 100 μL and pH=7 water 19.86mL, No. 3, add 100 μg / mL Chitosan-multi-walled carbon nanotube dispersion 200 μL and pH=7 water 19.76mL, No. 4, add 100 μg / mL chitosan-multi-walled carbon nanotube dispersion 400 μL and pH=7 water 19.56mL, Add 800 μL of 100 μg / mL chitosan-multi-walled carbon nanotube dispersion and 19.16 mL of water with pH=7, so that the concentration of the adsorbent in the mixed solution No. 1-5 is: 0.25 μg / mL, 0.50 μg / mL, 1.00μg / mL, 2.00μg / mL, 4.00μg / mL.

[0052] 1.2. Put the Erlenmeyer flask containing the...

Embodiment 2

[0066] Embodiment 2. investigate the enrichment effect under the different additions of chitosan dispersion liquid

[0067]2.1 Take 4 clean 500mL Erlenmeyer flasks with stoppers, numbered 1, 2, 3, 4, add 40 μL of 50 μg / mL reference substance mixture and 100 μL of chitosan-multi-walled carbon nanotube dispersion solution of 100 μg / mL, Add 50 μL of 100 μg / mL chitosan dispersion and 19.81 mL of pH=7 water to No. 1, add 100 μL of 100 μg / mL chitosan dispersion and 19.76 mL of pH=7 water to No. 3, add 100 μg / mL of water to No. 3 mL of chitosan dispersion 150 μL and pH=7 water 19.71mL, No. 4 was added 100 μg / mL chitosan dispersion 200 μL and pH=7 water 19.66mL, so that additional The concentration of chitosan added is as follows: 0.25 μg / mL, 0.50 μg / mL, 0.75 μg / mL, 1.00 μg / mL.

[0068] 2.2. Put the Erlenmeyer flask containing the mixed solution into the gyratory shaker and shake for 3 minutes. Use a 20mL disposable syringe to draw the solution in the Erlenmeyer flask.

[0069] 2.3...

Embodiment 3

[0077] Example 3. Investigate the enrichment effect of different shaking enrichment time

[0078] 3.1 Take 5 clean 50mL Erlenmeyer flasks with stoppers, numbered 1-5, add 19.86mL of water with pH=7, 40μL of 50μg / mL reference substance mixture, and 100μg / mL chitosan-multi-walled carbon nanotubes to disperse Solution 100μL.

[0079] 3.2. Put the Erlenmeyer flask containing the mixed liquid into the rotary oscillator, and shake it for 1 minute on No. 1, 3 minutes on No. 2, 5 minutes on No. 3, 7 minutes on No. 4, and 9 minutes on No. 5. Use a 20mL disposable syringe to draw the solution in the Erlenmeyer flask.

[0080] 3.3 Remove the needle, install a 0.22 μm filter head, place the syringe on a solid-phase extraction apparatus for suction filtration until there is no water drop, and remove the filter head containing multi-walled carbon nanotubes.

[0081] 3.4 Elute the filter head with 100 μL of methanol, collect the washing solution in a 1.5mL centrifuge tube, corresponding to...

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Abstract

The invention provides a method for enriching and detecting low-concentration phenol effective components. The method comprises the following steps: by taking a chitosan-multiwalled carbon nanotube dispersion liquid as an adsorbent, carrying out extraction-adsorption on a to be-detected sample liquid containing the low-concentration phenol effective components; then, eluting by using an eluent, wherein the eluent is the enriched sample liquid; detecting the enriched sample liquid by using liquid chromatograph / mass spectrometer and quantifying through a standard curve and an enrichment factor to detect the concentration of the phenol effective components in the be-detected sample liquid. The method provided by the invention can be used for detecting the low-concentration phenol effective components in a chrysanthemum diluent.

Description

technical field [0001] The invention belongs to the field of extraction and preparation of natural medicines. It involves a new, high-sensitivity extraction and enrichment technology for the enrichment and detection of low-concentration phenolic active ingredients. Background technique [0002] There are many chemical components with biological activity in plants, so their extraction process has attracted extensive attention. At present, the common techniques for extracting phytochemical components include reflux extraction, ultrasonic extraction, liquid-liquid microextraction, solid phase microextraction, microwave extraction, enzyme extraction, etc. Among them, solid-phase microextraction technology is a new extraction and separation technology developed on the basis of solid-phase extraction, which integrates sampling, pre-concentration, micro-extraction, and sample injection. Compared with common separation techniques, it has the advantages of simple and fast operation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/08G01N30/02
Inventor 曹君曹婉胡帅帅徐菁菁
Owner HANGZHOU NORMAL UNIVERSITY
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