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Traditional Chinese medicine composition for inhibiting microcystis aeruginosa

A technology of Microcystis aeruginosa and Microcystis green liquid, which is applied in the direction of chemicals, biocides, animal repellants, etc. for biological control, and can solve the problems of destroying the ecological landscape of water areas, liver damage, and death of aquatic organisms, etc. problems, to achieve the effect of improving the ecological environment of water bodies, low cost, and reducing the quantity

Inactive Publication Date: 2015-05-06
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Eutrophication of water body is one of the outstanding water environment problems at present, and one of its direct consequences is to cause cyanobacteria bloom. "Green Tide", the outbreak rate of Microcystis aeruginosa is extremely high, and it can produce a cyclic heptapeptide microcystin (Microcystins), which can seriously damage the liver of aquatic animals and can accumulate in the gonads of aquatic animals. A large number of aquatic organisms will die, which will not only seriously deteriorate the water quality, but also destroy the ecological landscape of the water area, destroy the balance and self-regulation ability of the ecosystem, affect fishery production, and even pass through the food chain, threatening human health

Method used

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  • Traditional Chinese medicine composition for inhibiting microcystis aeruginosa
  • Traditional Chinese medicine composition for inhibiting microcystis aeruginosa
  • Traditional Chinese medicine composition for inhibiting microcystis aeruginosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 500mL Erlenmeyer flask, (and then pour into the BG-11 medium (refer to the information of freshwater algae species bank for graduate students of aquatic biology in the Chinese Academy of Sciences) to make the initial growth of algae cells The density is 7.0×10 5 per milliliter, the volume is 200mL. Prepare different concentrations of algastatic agents (the weight ratio of Galla gall, Qinglongyi is 1:1), gallnut, Qinglongyi, and measure their inhibition rate to Microcystis aeruginosa. The results are shown in Tables 1, 2, and 3:

[0053] Table 1 Inhibition rate of gallnut and Qinglongyi combined on Microcystis aeruginosa (%)

[0054]

[0055] The data in the table are mean±SE (n=3).

[0056] Table 2 Inhibition rate (%) of gallnut on Microcystis aeruginosa

[0057]

[0058] The data in the table are mean±SE (n=3).

[0059] Table 3 Inhibition rate of Qinglongyi on Microcystis a...

Embodiment 2

[0067] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 50mL Erlenmeyer flask, and then pour into the medium to make the initial density of algal cells 7.0×10 5 per milliliter, the volume is 20mL. Algicides of different concentrations were prepared respectively (the weight ratio of Galla gall, Qinglongyi is 1:1) to measure its influence on the protein content of Microcystis aeruginosa algae liquid, and the results are shown in Table 5:

[0068] Table 5 Effects of gallnut and Qinglongyi compositions on the protein content of Microcystis aeruginosa liquid

[0069]

[0070] The data in the table are mean±SE (n=3).

[0071] *Compared with the control group at the same period, P<0.05; **Compared with the control group at the same period, P<0.01.

[0072] It can be seen from Table 5 that the protein content of algae liquid of Microcystis aeruginosa increases with the increase of concentration under the combined action of Galla chinensis and...

Embodiment 3

[0074] Pour the algae liquid of Microcystis aeruginosa in the logarithmic growth phase into a 500mL Erlenmeyer flask, and then pour into the culture medium so that the initial density of the algal cells is 7.0×10 5 per milliliter, the volume is 200mL. Prepare different concentrations of algastatic agents (the weight ratio of Galla gall, Qinglongyi is 1:1) to measure its influence on the nucleic acid content of Microcystis aeruginosa algae liquid, and the results are as shown in table 6:

[0075] Table 6 Effect of Galla galla and Qinglongyi composition on the nucleic acid content of Microcystis aeruginosa liquid

[0076]

[0077] The data in the table are mean±SE (n=3).

[0078] *Compared with the control group at the same period, P<0.05; **Compared with the control group at the same period, P<0.01.

[0079] Table 6 shows the changes in the nucleic acid content of Microcystis aeruginosa liquid. It can be seen from the table that the increase of the concentration of tradit...

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Abstract

The invention relates to a traditional Chinese medicine composition for inhibiting microcystis aeruginosa. The traditional Chinese medicine composition comprises Chinese gall and cortex juglandis mandshuricae according to a weight ratio of 1: 1. In a microcystis aeruginosa liquid, the inhibitor has an appropriate concentration scope of 9-9999mg / L. An appropriate microcystis aeruginosa density range is 1.0*10<5> to 2.5*10<7> per milliliter. Compared with the existing preparation for inhibiting microcystis aeruginosa, through combination of Chinese gall and cortex juglandis mandshuricae, the traditional Chinese medicine composition provided by the invention can substantially inhibit microcystis aeruginosa growth, reduce the content of microcystis aeruginosa in the microcystis aeruginosa liquid, and improve water quality and a water ecological environment. The traditional Chinese medicine composition utilizes natural products of Chinese gall and cortex juglandis mandshuricae, utilizes Chinese gall and cortex juglandis mandshuricae secondary metabolism products with allelopathic effects to inhibit microcystis aeruginosa growth, and has the characteristics of low cost, environmental friendliness and ecological safety.

Description

technical field [0001] The invention belongs to the field of algae inhibitors, and relates to an algae inhibitor for Microcystis aeruginosa, in particular to a traditional Chinese medicine composition capable of inhibiting Microcystis aeruginosa. Background technique [0002] At present, the main research direction of using allelochemical substances to inhibit algal blooms and granulate eutrophic water bodies. Since allelochemicals are secondary metabolites produced during plant growth, they are considered to be degradable in the natural environment and will not accumulate in the ecosystem for a long time, so they have good ecological security. [0003] Microcystic aeruginosa is the main algae of eutrophic cyanobacteria blooms in lakes, reservoirs and other water ecosystems in China. Eutrophication of water body is one of the outstanding water environment problems at present, and one of its direct consequences is to cause cyanobacteria bloom. "Green Tide", the outbreak rat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01P13/02C02F1/50
Inventor 聂江力裴毅
Owner TIANJIN AGRICULTURE COLLEGE
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