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Method for extracting total anthraquinones in rhubarb

A technology of total anthraquinone and rhubarb, which is applied in the field of extracting active ingredients in rhubarb, can solve the problems of low proportion of bound anthraquinone, easy hydrolysis, and difficult operation, etc., to increase the proportion of bound anthraquinone, shorten the production cycle, The effect of easy operation

Inactive Publication Date: 2015-05-06
NANTONG THIRD PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Second, the bound anthraquinone is easily hydrolyzed and will be converted into free anthraquinone during the extraction process
The extraction and separation process in the prior art is relatively complicated, which easily leads to the desugaring of the bound anthraquinone into its aglycone (free anthraquinone), and most of the final products are free anthraquinones, which leads to the extraction of the total anthraquinone in rhubarb. Low proportion of conjugated anthraquinone
[0007] Judging from the current technologies involving the extraction of total anthraquinones from rhubarb, there are many problems such as cumbersome process, difficult operation, serious damage to combined anthraquinones, or both.

Method used

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  • Method for extracting total anthraquinones in rhubarb
  • Method for extracting total anthraquinones in rhubarb
  • Method for extracting total anthraquinones in rhubarb

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Take 50 g of rhubarb palmatum coarse powder produced in Lixian County, Gansu, add 5 times the amount of 55% ethanol, heat and reflux for extraction 5 times, each time for 30 minutes, combine the ethanol extracts, recover ethanol under reduced pressure, and properly concentrate to obtain an extract. After the extract is cooled, add 10 times the amount of deionized water, stir evenly, and centrifuge at 5000 rpm for 10 minutes to obtain supernatant A and precipitate A. Concentrate the supernatant A properly, let it cool, add 95% ethanol to make the alcohol content reach 80%, add 10% sodium hydroxide solution until no precipitation occurs, centrifuge at 5000 rpm for 10 minutes, take the supernatant and wash it with hydrochloric acid Adjust the pH to 6.0-7.0, recover ethanol under reduced pressure, adjust the pH to 1.0-2.0 with hydrochloric acid after cooling, refrigerate at 4°C for 24-48 hours, filter with suction, and dry the filter cake in vacuum to obtain bound anthraquin...

Embodiment 2

[0034] Take 50g of rhubarb palmate powder, add 3 times the amount of 75% ethanol, heat and reflux for extraction 4 times, 45 minutes each time, combine the ethanol extracts, recover ethanol under reduced pressure, and properly concentrate to obtain the extract, add the extract after cooling 10 times the amount of deionized water, stirred evenly, and centrifuged at 5000 rpm for 10 minutes to obtain supernatant A and precipitate A. Concentrate the supernatant A properly, let it cool, add 95% ethanol to make the alcohol content reach 80%, add 10% sodium hydroxide solution until no precipitation occurs, centrifuge at 5000 rpm for 10 minutes, take the supernatant and wash it with hydrochloric acid Adjust the pH to 6.0-7.0, recover ethanol under reduced pressure, adjust the pH to 1.0-2.0 with hydrochloric acid after cooling, refrigerate at 4°C for 24-48 hours, filter with suction, and dry the filter cake in vacuum to obtain bound anthraquinone; Add 4 to 5 times of 8% hydrochloric ac...

Embodiment 3

[0037] Take 50g of rhubarb palmate coarse powder, add 4 times the amount of 95% ethanol, heat and reflux for extraction 3 times, 60 minutes each time, combine the ethanol extract, recover ethanol under reduced pressure, and properly concentrate to obtain the extract, add the extract after cooling 10 times the amount of deionized water, stirred evenly, and centrifuged at 5000 rpm for 10 minutes to obtain supernatant A and precipitate A. Concentrate the supernatant A properly, let it cool, add 95% ethanol to make the alcohol content reach 80%, add 10% sodium hydroxide solution until no precipitation occurs, centrifuge at 5000 rpm for 10 minutes, take the supernatant and wash it with hydrochloric acid Adjust the pH to 6.0-7.0, recover ethanol under reduced pressure, adjust the pH to 1.0-2.0 with hydrochloric acid after cooling, refrigerate at 4°C for 24-48 hours, filter with suction, and dry the filter cake in vacuum to obtain bound anthraquinone; Add 4 to 5 times of 8% hydrochlo...

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Abstract

The invention provides a method for extracting total anthraquinones in rhubarb, which comprises the following steps: adding ethanol to extract rhubarb coarse powder; recovering ethanol from the ethanol extracting solution to obtain an extract; adding water to the extract, and precipitating; adding hydrochloric acid / chloroform into the precipitate under reflux, cooling to take the chloroform layer, and removing the chloroform to obtain free anthraquinones; concentrating the supernate, adding ethanol until the ethanol content is 80%, regulating the pH value with alkali to precipitate, regulating the pH value of the surplus solution to neutrality, removing the ethanol, regulating the pH value to 1.0-2.0, and refrigerating to precipitate to obtain binding anthraquinones; and uniformly mixing the free anthraquinones and binding anthraquinones to obtain the rhubarb total anthraquinones. The method provided by the invention simplifies the rhubarb total anthraquinone extraction technique, is simple to operate, and shortens the production cycle. The method can greatly retain the binding anthraquinones, and enhances the ratio of the binding anthraquinones in the rhubarb total anthraquinone extract.

Description

technical field [0001] The invention relates to a method for extracting effective components in rhubarb, in particular to a method for extracting total anthraquinones in rhubarb. Background technique [0002] Rhubarb is the dry root and rhizome of Rheum tanguticum Maxim.ex Balf., Rheum palmatum L. or medicinal rhubarb (Rheum officinale Baill.) of Polygonaceae. Rhubarb contains a variety of ingredients, including anthraquinones, tannins, butyrophenones, stilbene, etc., of which the content of anthraquinones and tannins is relatively high. Rhubarb anthraquinones are divided into two types: free type and combined type, which are collectively referred to as total anthraquinones in rhubarb. The content of total anthraquinones in rhubarb is about 2% to 5%, of which free type anthraquinones only account for 10% to 20%, and free type anthraquinones The quinones are mainly aloe-emodin, rhein, emodin, chrysophanol, and emodin methyl ether, and the bound anthraquinones are mainly the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/708A61K125/00
Inventor 窦志华施忠王陈萍乔进卞理刘青青陈志强
Owner NANTONG THIRD PEOPLES HOSPITAL
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