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Rapid identification of tomato powdery mildew resistance gene

A powdery mildew resistance gene, tomato technology, applied in genetic engineering, plant genetic improvement, microbial determination/inspection, etc., to achieve the effects of low efficiency, shortening breeding years, and improving breeding efficiency

Inactive Publication Date: 2015-05-06
JIANGSU CHANGSHU MODERN AGRI IND PARK DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the basic research of tomato is not deep enough, it is not only time-consuming to use these methods but also it is difficult to accurately clone these genes

Method used

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  • Rapid identification of tomato powdery mildew resistance gene
  • Rapid identification of tomato powdery mildew resistance gene
  • Rapid identification of tomato powdery mildew resistance gene

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Embodiment Construction

[0032] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. The method can quickly identify the tomato powdery mildew gene. The specific implementation process is as follows:

[0033] 1) Collection and identification of tomato MLO gene

[0034] In order to obtain all the MLO-type gene family members of tomato, we first constructed an HMM model based on the MLO-type genes of Arabidopsis thaliana, and the MLO gene sequences of resistance to powdery mildew of tomato, pea, pepper, rose, pepper, and lotus root. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325903, TC315944,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC283253,TC283383,TC28503...

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Abstract

Relating to plant comparative genomics, genetics bioinformatics and other subject knowledge, the invention belongs to the science field of plant biotechnologies, and discloses rapid identification of tomato powdery mildew resistance gene. The main steps include: 1) download of tomato whole genome sequences and acquisition of MLO type genes; 2) identification of MLO type genes; 3) MLO type gene phylogenetic relationship; and 4) comparison of MLO type powdery mildew genes. The invention effectively shortens the tomato powdery mildew gene mining cycle, and is conducive to rapid identification of powdery mildew genes. Corresponding co-segregation functional markers (SNP, SCAR etc.) are developed through the identified candidate powdery mildew genes, and the markers can be rapidly used for molecular marker-assisted selection of powdery mildew resistance genes, and the accuracy is high. By combining other disease-resistant gene molecular markers, a plurality of resistance breeding materials can be created, the breeding period can be shortened, and the breeding efficiency is improved, thereby laying the foundation for elaboration of tomato powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the tomato powdery mildew gene by using methods such as plant comparative genomics, genetics, bioinformatics, and candidate gene strategy by means of sequencing the whole genome sequence of tomato, and mainly involves the download of the whole genome sequence of tomato and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Tomato, also known as tomato and foreign persimmon, is a plant of the nightshade family Solanaceae. The fruit is rich in nutrition and has a special flavor. It can be eaten raw, cooked, processed into tomato sauce, juice or stored as a whole fruit. Tomato is one of the most widely cultivated fruit vegetables in the world. Tomatoes are sweet and sour in taste, cool in nature and slightly cold in nature. Can clear heat and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G06F19/24C12N15/29C07K14/415
CPCC07K14/415C12Q1/6895C12Q2600/156
Inventor 钱春桃其他发明人请求不公开姓名
Owner JIANGSU CHANGSHU MODERN AGRI IND PARK DEV