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Gene detection method for ovarian response related genes

A genome and reaction well technology, applied in the field of molecular biology, can solve the problems of low ovarian response, "resistance" or decreased sensitivity, and increased FSH and target cell thresholds, and achieve high detection accuracy and repeatability. Effect

Inactive Publication Date: 2015-05-06
上海中优医药高科技股份有限公司
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Problems solved by technology

[0010]A large number of studies have found that in the process of IVF ovulation induction, recipients with the SS680 genotype are prone to ovarian hyporesponse, and there may be a certain degree of "resistance" to the effect of FSH ” or decreased sensitivity, leading to an increase in the threshold of FSH and target cells, requiring more gonadotropins to achieve the same effect

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Embodiment Construction

[0053] The purpose of the present invention is to provide a method for detecting genes related to ovarian response, and to predict and evaluate the risk of female infertility in combination with the specific environmental exposure factors and lifestyle factors related to ovarian response in the Chinese population.

[0054] To achieve the above objectives, the present invention provides a method for detecting genes related to ovarian response. The specific steps are:

[0055] Step 1: Types of samples tested and sampling methods

[0056] Use a sampling swab to swab the left and right cheeks in the mouth at least 40 times up and down to ensure that sufficient cell samples are collected;

[0057] Step 2: Extraction method of genomic DNA

[0058] The genomic DNA of each oral epithelial cell sample is extracted by silica gel adsorption method, and the time is 2 hours to 2.5 hours. After electrophoresis detection, clear white bands can be seen with the naked eye to judge that the o...

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Abstract

The invention discloses a gene detection method for ovarian response related genes. The method is characterized in that by detecting and analyzing the gene carrying type of FSHR (Follicle Stimulating Hormone Receptor) gene SNPs (single nucleotide polymorph sims) loci of individuals, the detection on ovarian response related genes is provided for detected people so as to screen infertility susceptible individuals in Chinese female populations; therefore, on the basis of giving infertility attack risks of individuals, the chances of early knowing, preventing and treating infertility genetic susceptibility are provided for Chinese females.

Description

technical field [0001] The invention relates to a method for detecting genes related to ovarian response, which can be used for evaluating the detection of genes related to ovarian response, and belongs to the technical field of molecular biology. Background technique [0002] Infertility is a worldwide medical and social problem, and infertile couples account for 10% to 15% of married couples of childbearing age. There are many reasons for female infertility. Environmental factors and lifestyles have a significant impact on the occurrence of female infertility. Infertility caused by genetic factors is also common. Chromosomal abnormalities or gene defects can cause female infertility. These abnormalities or defects (including chromosomal diseases, single gene diseases, polygenic diseases, etc.) can be passed from parent to offspring, or formed by mutations in germ cells of offspring, which can lead to adverse pregnancy or infertility. [0003] In recent years, studies on f...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2563/107
Inventor 傅咏南王校张奕
Owner 上海中优医药高科技股份有限公司
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