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Method for isolating and identifying compound through fluorescent quenching

A fluorescence quenching and compound technology, applied in the field of fluorescence quenching separation and identification of compounds, can solve the problems of no fluorescence, colorless compounds, and difficult to measure.

Inactive Publication Date: 2015-05-06
SHANGHAI ZHONGHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Some compounds are colorless and non-fluorescent, and there is no suitable chromogenic agent to develop color with them, and there is no condition for generating fluorescent organisms, so it is difficult to determine

Method used

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  • Method for isolating and identifying compound through fluorescent quenching
  • Method for isolating and identifying compound through fluorescent quenching
  • Method for isolating and identifying compound through fluorescent quenching

Examples

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Embodiment 1

[0034] A method for fluorescence quenching separation and identification of compounds, taking the TLC identification method of cinnamaldehyde as an example.

[0035] Cinnamic aldehyde is a volatile component, and the optimal TLC identification method for cinnamic aldehyde was selected from the aspects of sample processing method, developer, and inspection method.

[0036] 1. Selection of the preparation method of the test solution

[0037] Method 1: Take an appropriate amount of this product, mix it evenly, take 0.2g, accurately weigh it, put it in a 25ml measuring bottle, add 10ml of petroleum ether (60-90°C), heat it on a 50°C water bath for several minutes to melt, let it cool, Add petroleum ether (60-90°C) to dilute to the mark, shake well, centrifuge, and take the supernatant to obtain the product. The sample volume is 5 μl.

[0038] Method 2: The preparation method of the test sample was prepared by using the thin-layer identification method of cinnamaldehyde in the qu...

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Abstract

The invention discloses a method for isolating and identifying a compound through fluorescent quenching. The method comprises the following steps: (1) preparation of control liquid: taking a control substance, adding petroleum ether at 60-90DEG C to prepare a liquid of 0.5g per 1ml, as a control substance liquid; (2) preparation of test article liquid: taking a proper amount of samples, evenly mixing, taking 0.2g, precisely weighting, placing into a 25ml measuring flask, adding 10ml of petroleum ether at 60-90DEG C, heating for minutes on a 50DEG C water bath and thawing, cooling, adding 10ml of petroleum ether at 60-90DEG C and diluting to the scale, shaking, centrifuging and taking supernatant; (3) preparation of a developer: preparing petroleum ether -ethyl acetate at 60-90DEG C; and (4) thin layer chromatography conditions: performing sample application and developing on a GF254 thin layer plate; and (5) inspection: taking out, air-drying, and inspecting under an ultraviolet lamp at 254nm.

Description

technical field [0001] The invention relates to a method for separating compounds, in particular to a method for fluorescence quenching separation and identification of compounds. Background technique [0002] Thin-layer chromatography, thin layer chromatography in English, one of the chromatography methods, is an important experimental technique for rapid separation and qualitative analysis of a small amount of substances. It belongs to solid-liquid adsorption chromatography and has both the advantages of column chromatography and paper chromatography. On the one hand, it is suitable for the separation of a small amount of samples (several to several micrograms, even 0.01 micrograms); on the other hand, when making thin-layer plates, the adsorption layer is thickened and enlarged, so it can be used to refine samples. This method is especially suitable for volatilization Substances that are less stable or prone to change at higher temperatures cannot be analyzed by gas chrom...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 金家骅丁礼琴鞠家星
Owner SHANGHAI ZHONGHUA PHARMA
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