Method for reducing content of protein in sodium hyaluronate prepared by bio-extraction method and prepared sodium hyaluronate

A technology for protein content and sodium hyaluronate is applied in the field of reducing the protein content in the preparation of sodium hyaluronate by biological extraction method and the field of the prepared sodium hyaluronate, which can solve the problems of increasing production costs and failing to meet the quality standards of sodium hyaluronate, and achieves a reduction in the production of sodium hyaluronate. Production cost, lower protein content higher effect

Inactive Publication Date: 2015-05-13
SHANGHAI JINGFENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the protein content of sodium hyaluronate produced by the traditional process is about 0.40-0.80%, which is relatively high, and cannot meet the new quality standard of sodi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Get 2kg of medicinal solution, add CPC (cetylpyridinium chloride) 6.5g therein, carry out complexation precipitation, precipitate is rinsed 5 times, then dissociate with 0.3mol / L sodium chloride solution, dissociation is complete Finally, dilute the dissociation solution with purified water to a conductivity of 7.0ms / cm, then discharge the supernatant, rinse the precipitate 3 times, and dissociate with 0.3mol / L sodium chloride solution, then filter, Ethanol precipitation, vacuum drying to obtain sodium hyaluronate B1. The content of protein in sodium hyaluronate was determined by Folin's phenol method.

Embodiment 2

[0030] Get 2kg of medicinal solution, add CPC (cetylpyridinium chloride) 6.5g therein, carry out complexation precipitation, precipitate is rinsed 5 times, then dissociate with 0.3mol / L sodium chloride solution, dissociation is complete Finally, dilute the dissociation solution with purified water to a conductivity of 7.5ms / cm, then discharge the supernatant, rinse the precipitate 5 times, and dissociate with 0.3mol / L sodium chloride solution, then filter, Ethanol precipitation, vacuum drying to obtain sodium hyaluronate B2. The content of protein in sodium hyaluronate was determined by Folin's phenol method.

Embodiment 3

[0032] Get 2kg of medicinal solution, add CPC (cetylpyridinium chloride) 6.5g therein, carry out complexation precipitation, precipitate is rinsed 5 times, then dissociate with 0.3mol / L sodium chloride solution, dissociation is complete Finally, dilute the dissociation solution with purified water to a conductivity of 8.0ms / cm, then discharge the supernatant, rinse the precipitate 5 times, and dissociate with 0.3mol / L sodium chloride solution, then filter, Ethanol precipitation, vacuum drying to obtain sodium hyaluronate B3. The content of protein in sodium hyaluronate was determined by Folin's phenol method.

[0033] Comparative example and embodiment detection data summary are as shown in table 1 as follows:

[0034] Table 1

[0035] sample

[0036] As can be seen from the test data in Table 1, the method used in the present invention can significantly reduce the protein content in the sodium hyaluronate produced by the biological extraction method.

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Abstract

The invention relates to a method for reducing the content of protein in sodium hyaluronate prepared by a bio-extraction method. At present, a comb extract method for producing the sodium hyaluronate usually comprises the following steps: coarse cleaning, fine cleaning and grinding, enzymolysis, primary adsorption, CPC precipitation and dissociation, secondary adsorption, alcohol precipitation, and drying to obtain sodium hyaluronate crude drug. According to the method, after performing the CPC precipitation and dissociation, purified water is added to a dissociation solution, so that the ion strength of the dissociation solution is reduced; based on the quaternary ammonium salt complex-precipitation dissociation principle, positive ions on a quaternary ammonium group is free under the low salt concentration and is combined with negative ions on a mucopolysaccharide molecule to form a quaternary ammonium complex, and the quaternary ammonium complex is gradually dissociated under the high salt concentration. By virtue of the reversible reaction, precipitates in the dissociation solution are repeatedly complexed, and then are subjected to the steps of rinsing, secondary dissociation, filtering, alcohol precipitation and drying so as to obtain the sodium hyaluronate crude drug. According to the method, the content of protein in the sodium hyaluronate can be remarkably reduced.

Description

Technical field: [0001] The invention relates to a method for reducing protein content in sodium hyaluronate prepared by biological extraction. Background technique: [0002] Sodium hyaluronate is a linear polysaccharide composed of (1→3)-2-acetylamino-2-deoxy-β-D-glucose-(1→4)-O-β-D glucuronic acid disaccharide repeating unit. glycans. The structure of HA is very regular, the same disaccharide unit is the basic structural unit of HA, and there is no species difference in HA from different animal tissues and bacteria. Non-antigenic to humans and animals. [0003] The chain length and amount of HA are not uniform. The amount ranges from 2×105 to 7×106, and the length of disaccharide units is 300-11000 pairs, which belongs to biological macromolecules. Commercial HA is generally in the form of sodium salt, a white fibrous or powdery solid, with strong hygroscopicity, soluble in water, insoluble in organic reagents, the medical fraction is 1×106~2.5×106, and the content is 9...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 叶湘武汪洋黄敏敏钱正祥
Owner SHANGHAI JINGFENG PHARMA
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