Reflective split-pupil confocal-photoacoustic microscopy imaging device and method
A photoacoustic microscopy and imaging device technology, applied in the direction of material excitation analysis, etc., can solve the problems of inability to realize cell structure images, etc., and achieve the effects of easy miniaturization and hand-held, increased working distance, and improved axial resolution
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Embodiment 1
[0037] The embodiment of the present invention is based on Figure 4 The reflective split-pupil confocal-photoacoustic microscopy imaging device shown includes a point light source system 1 composed of a laser 14, a focusing mirror 13 and a first pinhole 12, and a collimating mirror 3 placed in the direction of travel of the pulse beam 2 in sequence , a reflective condenser 4, an aberration compensating hemispherical mirror 7, a three-dimensional beam scanner 8 and a sample to be tested 11, and an acoustic lens 6 and an ultrasonic transducer 5 that are placed in the backscattering direction of the sample 11 to detect photoacoustic signals in sequence, And a reflective split-pupil confocal detection system for detecting the backscattered light or fluorescence signal of the sample under test 11; wherein, the reflective split-pupil confocal detection system includes: aberrations placed in sequence along the backscattering direction of the measured sample Compensation hemispherica...
Embodiment 2
[0070] Such as figure 2 As shown, in the reflective split-pupil confocal-photoacoustic microscopy imaging device of Embodiment 1, the point light source system 1 is replaced by an exit optical fiber 17 . The pulse beam 2 emitted by the output fiber 17 is focused on the sample 11 to excite a photoacoustic signal, scattered light or fluorescence signal after being passed through the collimating mirror 3, the reflective condenser mirror 4, and the aberration compensating hemispherical mirror 7.
[0071] In the reflective split-pupil confocal-photoacoustic microscopy imaging device of Embodiment 1, the point detector 10 is replaced by a detection fiber 18 . The scattered light or fluorescent signal of the measured sample excited by the focused beam is focused to the detection fiber 18 for detection through the aberration compensating hemispherical mirror 7, the three-dimensional beam scanner 8, the reflecting condenser mirror 4, and the collecting mirror 9.
[0072] All the othe...
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