Fingerprint detection method of Xiaokeqing preparation

A technology of fingerprint spectrum and detection method, which is applied in the field of determination of active ingredients of medicines, can solve the problems of complex chemical composition of traditional Chinese medicine, unclear mechanism of action, and difficulty in ensuring the quality and stability of traditional Chinese medicine products.

Active Publication Date: 2015-05-13
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the complex chemical composition and unclear mechanism of action of traditional Chinese medicine, it is difficult to ensure the quality stability of traditional Chinese medicine products in the existing traditional Chinese medicine pharmaceutical technology.

Method used

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  • Fingerprint detection method of Xiaokeqing preparation
  • Fingerprint detection method of Xiaokeqing preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0148] The ultra-high performance liquid phase standard control fingerprint of Xiaokeqing granule is obtained as follows:

[0149] 1) Preparation of the test solution:

[0150] Remove 6g of Keqing granules, put them in a 25ml measuring bottle, add an appropriate amount of 75% methanol, ultrasonicate at a power of 500w for 30min, place it at room temperature, add 75% methanol to the volume, filter through a 0.22μm microporous membrane, and take the subsequent filtrate, namely have to;

[0151] 2) Obtain the chromatogram:

[0152] The test solution is injected into an ultra-high performance liquid chromatograph to obtain a chromatogram, wherein the reference peaks are mangiferin peaks and timosaponin BII peaks;

[0153] 3) Generate a control fingerprint

[0154] Select qualified multiple batches of Xiaokeqing preparation chromatograms and import them into the Chinese Pharmacopoeia Commission's "Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation Sy...

Embodiment 2

[0168] The ultra-high performance liquid phase standard control fingerprint of Xiaokeqing preparation is obtained as follows:

[0169] 1) Preparation of the test solution:

[0170] Cancel the thirst-clearing preparation, add a certain proportion of methanol or ethanol aqueous solution of the extraction solvent, ultrasonically dissolve, then add a solvent to adjust the solution concentration, filter through a microporous membrane, take the filtrate, and obtain it;

[0171] 2) Obtain the chromatogram:

[0172] The test solution is injected into an ultra-high performance liquid chromatograph to obtain a chromatogram;

[0173] 3) Generate a control fingerprint

[0174]Select qualified multiple batches of Xiaokeqing preparation chromatograms and import them into the Chinese Pharmacopoeia Commission's "Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System Version A" software to calculate the similarity results and generate standard control fingerpri...

Embodiment 3

[0194] The ultra-high performance liquid phase standard control fingerprint of Xiaokeqing preparation is obtained as follows:

[0195] 1) Preparation of the test solution:

[0196] Take 1-7g of Qukeqing preparation, use 50-80% ethanol or 25-80% methanol for ultrasonic extraction, preferably 75% ethanol, or 50-75% methanol, and the ultrasonic time is 20-40min;

[0197] 2) Obtain the chromatogram:

[0198] The test solution is injected into an ultra-high performance liquid chromatograph to obtain a chromatogram;

[0199] 3) Generate a control fingerprint

[0200] Select qualified multiple batches of Xiaokeqing preparation chromatograms and import them into the Chinese Pharmacopoeia Commission's "Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System Version A" software to calculate the similarity results and generate standard control fingerprints;

[0201] The chromatographic conditions are as follows:

[0202] Chromatographic column: choose UP...

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Abstract

The invention relates to a fingerprint detection method of a Xiaokeqing preparation. The method comprises the following steps: 1, preparing the chromatogram of a sample to be detected: taking a Xiaokeqing preparation sample, adding an extract solvent which is an aqueous solution containing a proportion of methanol or ethanol, carrying out ultrasonic dissolving, adding the solvent to adjust the concentration of the obtained solution, filtering through a millipore filtration membrane, taking the obtained filtrate, and injecting the filtrate to an ultrahigh performance liquid chromatograph to obtain the chromatogram; and 2, calculating the similarity of the chromatogram of the sample to be detected and the a standard control fingerprint: introducing the chromatogram integration signal of the sample to be detected into software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version A) published by Chinese Pharmacopoeia Commission, and comparing the similarity of the chromatogram of the sample to be detected with the similarity of the standard control fingerprint.

Description

Technical field: [0001] The invention relates to a method for measuring active ingredients of medicines, in particular to a method for detecting fingerprints of Xiaokeqing preparations. Background technique: [0002] Xiaokeqing granule is a Chinese patent medicine for treating diabetes already on the market. Its formula is composed as follows: Anemarrhena anemarrhena, Atractylodes atractylodes, Coptis chinensis, Puhuang, Dijincao, and appropriate amount of auxiliary materials. The preparation method includes the following steps: [0003] (a) Anemarrhena, Atractylodes atractylodes, Coptis chinensis, Puhuang, Dijincao are taken for use; [0004] (b) Atractylodes atractylodis extracts volatile oil by steam distillation for 5 hours, then clathrates atractylodes volatile oil with β-cyclodextrin, feeds atractylodes atractylodis oil and β-cyclodextrin at a ratio of 1:6, and dries at 50°C to obtain β-cyclodextrin The fine clathrate is set aside, and the extract is filtered to obtai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 佟玲周水平孙鹤李东翔王露黔李晓稳
Owner TIANJIN TASLY PHARMA CO LTD
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