Feed capable of improving anti-stress capability of large yellow croakers
A large yellow croaker and feed technology, applied in animal feed, animal feed, application, etc., can solve the problems of acute death of large yellow croaker, nutritional changes of large yellow croaker, etc.
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Embodiment 1
[0015] Embodiment 1: Application of large yellow croaker anti-stress feed formula
[0016] The main raw materials are fish meal, soybean meal, wheat flour, fish oil, soybean lecithin, large yellow croaker mineral premix, large yellow croaker vitamin premix (without vitamin C), choline chloride, calcium dihydrogen phosphate, and betaine. Two kinds of compound feeds for large yellow croaker (see Table 1 for details): 1# feed is the treatment group that adds the amount of vitamin C phosphate (283 mg / kg) just enough to meet the growth needs of large yellow croaker; 2# feed is added to meet the growth needs 4 times The treatment group of the amount of vitamins.
[0017] Table 1: Experimental Feed Formula
[0018]
[0019] Among them, mineral premix (mg / kg): magnesium sulfate heptahydrate (MgSO 4 .7H 2 O), 182.57; copper sulfate pentahydrate (CuSO 4 .5H 2 O), 0.98; ferrous sulfate heptahydrate (FeSO 4 .7H 2 O), 11.95; zinc sulfate heptahydrate (ZnSO 4 .7H 2 O), 7.59; man...
Embodiment 2
[0040] Other operations are the same as in Example 1.
[0041] Determination of total protein content, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activity in the serum of large yellow croaker before and after two kinds of stress experiments in the two treatment groups, so as to reflect the effect of high content of vitamin C in the feed on fish liver. side effects. The total protein content, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activity and other indicators were determined by kits produced by Nanjing Jiancheng Bioengineering Technology Research Institute.
[0042] The experimental results are represented by mean ± standard deviation (mean ± S.D.). There were significant differences (P<0.05) in Tukey's multiple comparisons among means with different superscripts in the same data row.
[0043] Table 4: Effects of excessive vitamin C supplementation on liver enzyme activity of large yellow croaker
[0044] ...
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