Process technology of extracting crocin-type component from gardenia

Abstract

The invention discloses a process technology of extracting a crocin-type component from gardenia. The process technology includes following steps: (1) crushing gardenia fruit into crude powder and placing the crude powder in an ultrasonic cleaning trough; (2) adding 75% ethanol by 8 times to the crude powder and performing ultrasonic alcohol extraction at a certain temperature for 1 h to obtain an extract; (3) filtering-out the extract, adding 75% ethanol by 6 times and performing ultrasonic alcohol extraction at a certain temperature for 1 h to obtain an extract to obtain an extract; (4) filtering-out the extract, mixing the two extracts and performing reduced-pressure concentration to the mixed extract in a rotary evaporator to a relative density of 1.15-1.20 to obtain a concentration liquid; (5) adding 80% ethanol by 4 times and refrigerating the concentration liquid for 10 h, filtering the concentration liquid to remove precipitation and performing reduced-pressure concentration to a supernate to the relative density of 1.15-1.20; (6) adding 80% ethanol by 8 times and refrigerating the concentration liquid for 10 h to obtain a precipitation; (7) dissolving the precipitation in a proper amount of water to obtain a water solution and feeding the water solution into macroporous resin columns comprising types of D101 and AB-8 in series, washing the water solution until the water solution is almost colorless with 15% ethanol and eluting the water solution with 85% ethanol; and (8) collecting an eluted liquid and reduced-pressure drying the eluted liquid to obtain the crocin-type component.

Description

technical field [0001] The invention relates to a method for extracting crocin components of gardenia jasminoides, which uses pulsed ultrasonic waves for alcohol extraction and macroporous resin series technology for adsorption and separation, and belongs to the field of biology and new medicine. Background technique [0002] Gardenia is derived from the ripe fruit of Gardenia jasminoides of the Rubiaceae plant. It is a traditional Chinese medicinal material abundant in my country. One of the glycoside components, crocin, is its active ingredient. Crocin was first extracted from saffron, a national precious medicinal material. Its outstanding curative effect of removing blood stasis and reducing blood fat has been widely recognized by the medical field. However, due to the high price and high extraction cost of saffron, it is not suitable for expanding production. Using gardenia fruit instead of saffron to extract saffron glycosides has become a major breakthrough in the his...

AI Technical Summary

Problems solved by technology

[0004] The use of macroporous adsorption resin technology for adsorption and separation has been reported in many literatures, but the use of D101 macroporous resin alone for adsorption and separation results in a low yield of crocin components