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Fermentation method for increasing yield of polysaccharide as active ingredient of biological flocculants

A technology of biological flocculants and active ingredients, which is applied in the field of fermentation to improve the active ingredients of biological flocculants, can solve problems such as instability, low output, and complex industrial fermentation processes, and achieve obvious effects, increase content, and increase activity.

Active Publication Date: 2015-06-03
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, there is a problem of low yield of bio-flocculants, which prevents the production of bio-flocculants in large quantities.
In particular, the complex and unstable characteristics of the industrial fermentation process make it impossible to predict the fermentation process

Method used

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  • Fermentation method for increasing yield of polysaccharide as active ingredient of biological flocculants
  • Fermentation method for increasing yield of polysaccharide as active ingredient of biological flocculants
  • Fermentation method for increasing yield of polysaccharide as active ingredient of biological flocculants

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specific Embodiment approach 1

[0012] Embodiment 1: A fermentation method for increasing the yield of polysaccharides, the active ingredient of a bioflocculant, according to this embodiment, is operated in accordance with the following steps: first, inoculate the bioflocculant producing bacteria into the flocculation fermentation liquid medium, and ferment to prepare the seeds liquid; then inoculate the seed liquid in the flocculation fermentation liquid medium added with exogenous AHLs, and carry out secondary fermentation to obtain the bioflocculant, and complete the fermentation of improving the polysaccharide production of the active ingredient of the bioflocculant; wherein , the exogenous AHLs are N-3-oxo-octanoyl homoserine lactone and N-hexanoyl homoserine lactone or one or both of them are mixed in any ratio; among them, the flocculation fermentation liquid added with exogenous AHLs The culture medium is to add exogenous AHLs into the flocculation fermentation liquid medium until the final concentrat...

specific Embodiment approach 2

[0013] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the bioflocculant-producing bacteria are Gram-negative bacteria. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0014] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the gram-negative bacteria is Agrobacterium tumefaciems (Agrobacterium tumefaciems) F2, which is preserved in China Microorganism Culture Collection Center, and the preservation address is Beichen West, Chaoyang District, Beijing No. 3, Courtyard No. 1, the preservation date is December 03, 2014, and its CGMCC number is 10131. Others are the same as in the first embodiment.

[0015] The bioflocculant-producing bacteria Agrobacterium tumefaciems F2 of this embodiment is a Gram-negative bacterium, and the bioflocculant produced by it is a metabolite of the bacterium, and its flocculation active ingredients mainly exist in the fermentation broth. The flocculation rate reaches more than 80%, and has good thermal stability; through experiments, it is confirmed that the polysaccharide in the biological flocculant of Agrobacterium tumefaciems (Agrobacterium tumefaciems) F2 is ...

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Abstract

The invention discloses a fermentation method for increasing the yield of polysaccharide as an active ingredient of biological flocclants, which relates to a fermentation method for increasing the active ingredient of the biological flocclants. The method comprises the following steps of firstly, inoculating flocculant-producing microorganisms into a flocculant fermentation fluid medium for fermentation to prepare seed solution; then inoculating the seed solution into the flocculant fermentation fluid medium which is added with exogenous AHLs for secondary fermentation to prepare the biological flocclants, so that the fermentation for increasing the yield of the polysaccharide as the active ingredient of the biological flocclants is finished; wherein the exogenous AHLs is one of N-3-oxygen-decoyl homoserine lactone and N- caproyl homoserine lactone or is a mixture of the same at any ratio. By adopting the method disclosed by the invention, the addition of the exogenous AHLs can improve the concentration and flocculation activity of the polysaccharide as the active ingredient of the biological flocclants, the flocculation effect of the biological flocculants is improved, and a basis is laid for large-scale industrial fermentation of the biological flocculants.

Description

technical field [0001] The invention relates to a fermentation method for increasing the active component of a biological flocculant. Background technique [0002] As the core technology of water treatment, the research and development of new products of water treatment materials has always represented the frontier of water treatment technology. Flocculant is one of the core technologies with good effect, and plays an important role in the most common and critical flocculation stage in water treatment. In today's water treatment, flocculants have been used on a large scale to treat urban domestic sewage and industrial wastewater, especially in the treatment of special wastewater. Among them, the emergence of biological flocculants solves the problems that traditional flocculants are not easy to biodegrade, and is easy to cause harm to the environment and health. It has the advantages of wide source, rich variety, non-toxicity, and good flocculation ability, so it is more an...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01
Inventor 李昂吴丹杨基先皮姗姗魏薇郭海娟庞长泷马放
Owner HARBIN INST OF TECH
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