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Neomycin and Tylosin two-in-one gold standard microporous test strip

A technology of tylosin and neomycin, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high technical requirements for operation, long operation time, complex processing, etc., and achieve broad market prospects, low cost, advanced Handle simple effects

Active Publication Date: 2016-08-31
北京维德维康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The detection methods of neomycin and tylosin reported at home and abroad mainly include high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), liquid chromatography-tandem mass spectrometry (LC-MS MS / MS) is the most commonly used method for detecting antibiotics, but in actual operation, high-end instruments such as LC-UV, LC-MS and LC-MS / MS are required, and the processing is complicated, cumbersome, long-term operation, and high-tech operation requirements
At present, there is no product that can simultaneously detect the residues of neomycin and tylosin

Method used

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  • Neomycin and Tylosin two-in-one gold standard microporous test strip
  • Neomycin and Tylosin two-in-one gold standard microporous test strip
  • Neomycin and Tylosin two-in-one gold standard microporous test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, the preparation of the colloidal gold marker of conjugate, specific antibody and specific antibody

[0060] 1. Preparation of conjugates

[0061] 1. Preparation of neomycin-carrier protein conjugates

[0062] (1) Take 20mg of neomycin API and dissolve it in 4ml of carbonate buffer (pH9.5, 0.1M).

[0063] (2) Take 60mg of BSA and dissolve it in 6ml of carbonate buffer (pH9.5, 0.1M).

[0064] (3) Add the solution obtained in step (1) dropwise to the BSA solution obtained in step (2), shake and mix at room temperature for 10 minutes, then add 325 μl of 1% (volume ratio) glutaraldehyde aqueous solution and stir at room temperature (400 rpm) overnight, Then add NaBH dropwise 4 solution (4mg dissolved in 400μl pure water, newly prepared) and stirred at room temperature for 1h, then placed in a dialysis bag and dialyzed with distilled water (dialysis process parameters: 4°C, 100rpm stirring, 2 days, changing the solution twice a day), and then with Dialyze wi...

Embodiment 2

[0101] Embodiment 2, the assembly of kit

[0102] figure 1 It is a schematic diagram of the structure of the test strip. figure 2 It is a schematic diagram of the use state of the kit. image 3 Schematic diagram for the interpretation of the results of the kit.

[0103] The kit for detecting neomycin and tylosin provided by the present invention includes the sample plate prepared in Step 3 of Example 1 and 8 identical test strips. The kit also includes an aluminum foil bag, and the sample plate and the test strip are packaged in the aluminum foil bag. Each test strip includes a bottom plate 1 , a reaction film 2 , a sample absorbent pad 3 and a water absorbent pad 4 . The sample absorbent pad, the reaction membrane and the water absorbent pad are attached to the bottom plate in sequence, the end of the sample absorbent pad is connected to the beginning of the reaction membrane, and the end of the reaction membrane is connected to the water absorbent pad. connected at the...

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Abstract

The invention discloses a neomycin and tylosin two-in-one gold-labeled microporous test paper strip. A kit provided by the invention comprises a sample board and at least one test paper strip, wherein the sample board is at least provided with one sample groove; the bottom of each sample groove is attached with a colloidal gold label of a neomycin specific antibody and a colloidal gold label of a tylosin specific antibody. Each test paper strip comprises a bottom plate (1), a reaction film (2), a sample absorption pad (3) and a water absorption pad (4), wherein the corresponding sample absorption pad, the corresponding reaction film and the corresponding water absorption pad are sequentially attached to the corresponding bottom plate; each reaction film is sequentially provided with a T2 line, a T1 line and a C line from the initial end to the tail end; the corresponding T2 line and the corresponding T1 line are respectively coated with the following two kinds of conjugates, namely a tylosin-carrier protein conjugate and a neomycin-carrier protein conjugate; the corresponding C line is coated with an antiantibody. The kit provided by the invention has a broad market prospect and relatively great economic and social benefits.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a neomycin and tylosin two-in-one gold standard micropore for simultaneous detection of neomycin and tylosin residues in animal-derived foods (such as milk, etc.) test strips. Background technique [0002] Neomycin (neomycin, NEO) belongs to the aminoglycoside antibiotics, which is composed of neomycin and neomycobiosamine. Among them, neomycin is formed by combining D-glucodiamine and deoxystreptamine, and neomycosamine is formed by combining D-ribose and neomycosamine. Neomycin is a mixture of two stereoisomers neomycin B and neomycin C, in which neomycin B is the main component, and neomycin sulfate is often used clinically. Neomycin is an important anti-infective drug with a broad antibacterial spectrum, and has good inhibitory effect on Gram-negative bacteria, Gram-positive bacteria and Mycobacterium tuberculosis. In veterinary clinical practice, neomycin ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/531G01N33/558
Inventor 李向梅吴小平王世恩王照鹏温凯王文珺许舒婷李宁
Owner 北京维德维康生物技术有限公司