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Cordyceps sinensis black rice

A technology for black rice and Cordyceps militaris, applied in the field of bioengineering, can solve problems such as affecting the significant efficacy of Cordyceps sinensis, and achieve the effects of being easy to realize large-scale production, wide application prospects, and simple and feasible methods

Inactive Publication Date: 2015-06-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interestingly, cordycepin is mainly present in Cordyceps militaris, and its content in Cordyceps sinensis is very small, but the absence of cordycepin did not affect the significant efficacy of Cordyceps sinensis

Method used

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  • Cordyceps sinensis black rice
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Examples

Experimental program
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Effect test

Embodiment 1

[0018] The DS56-Ⅲ twin-screw extruder produced by Jinan Juxian Machinery Equipment Co., Ltd. was used to process black rice. The black rice used was commercially available, and its moisture content was 14.7% as determined by a moisture analyzer. The feed rate was set at 120 kg / h. Table 1 lists the temperature combination and expansion degree of the three sections. It can be seen from Table 1 that when the temperatures in the preheating zone, transportation zone, and ripening zone are 60, 160, and 190°C respectively, the degree of puffing of black rice is the highest. The black rice was further dried to investigate the puffing degree at different water contents. As can be seen from Table 2, the puffing effect is the best when the water content is about 14-18%, so commercially available black rice can be directly used for puffing.

[0019] Table 1: The puffing degree of puffed black rice obtained by the combination of different temperatures in different sections of the extrusi...

Embodiment 2

[0024] Inoculate the Cordyceps sinensis strain into a 250ml Erlenmeyer flask with 50ml medium, the composition of the medium is: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, culture for 96 hours , as seeds for inoculation.

[0025] Inoculate the Cordyceps militaris strain into a 250ml Erlenmeyer flask with 50ml of culture medium. The culture medium is composed of: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, and culture for 96 hours as seeds for inoculation.

[0026] Mix puffed black rice with a puffing degree of 2.5 and black rice with a mechanical crushing degree of 1mm, totaling 50g, add 40g of distilled water, put it into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, and inoculate Cordyceps sinensis and Cordyceps militaris seeds after cooling 5ml of each solution, in a constant temperature and humidity incubator, control the ambient humidity to 90%, the temperature is 25°C, and measure the content of ...

Embodiment 3

[0030] Mix 30g of puffed black rice with a puffing degree of 2.5 and 20g of black rice with a mechanical crushing degree of 2mm, add 40g of distilled water, put it into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, and inoculate the cultured Cordyceps sinensis and pupae after cooling Each 5ml of Cordyceps seed solution was controlled in a constant temperature and humidity incubator with an ambient humidity of 80% and a temperature of 15°C. After 20 days of fermentation, the cordycepin content was determined to be 5.2%.

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Abstract

Cordyceps sinensis black rice takes black rice as a culture medium; cordyceps sinensis and cordyceps militaris are subjected to double-bacteria mixed solid-state fermentation, and the cordyceps sinensis black rice belongs to the technical field of biological engineering. The black rice obtained by double-bacteria bioconversion has life cultivation and health preservation effects and has a good application prospect.

Description

technical field [0001] The method adopts Ophiocordyceps sinensis and Cordyceps militaris, uses black rice as a culture medium, and performs double-bacteria mixed solid-state fermentation, and belongs to the technical field of bioengineering. The preservation number of the bacterial strain Cordyceps militaris is CICC 14013, and the preservation number of the bacterial strain Ophiocordyceps sinensis is CICC 14088, and the nutrition and health black rice is obtained through double-bacteria mixed solid-state fermentation. Background technique [0002] Cordyceps belongs to Fungi, Ascomycota, Ascomycetes, Sordariomycetidae, Hypocreales, Clavcipitaceae . Most of the fungi of the genus Cordyceps are medicinal fungi, among which Ophiocordyceps sinensis is generally known and has the longest application history, and Cordyceps militaris has the most in-depth application research. [0003] According to literature research, Cordyceps sinensis was first recorded in "Yuewang Yaozheng" in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05F11/00
Inventor 蔡宇杰杨腾义白玉管政兵廖祥儒张大兵
Owner JIANGNAN UNIV
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