Breeding method of basic nutritional cytoplasmic male sterile line of stingless purple flowers of hibiscus cannabinus

A male sterile line, nutritional technology, applied in the field of crop genetics and breeding, can solve the problems of decreased biological yield and fiber yield, and achieve the effect of alleviating the pain of skin, ensuring production safety, and ensuring the purity of germplasm.

Inactive Publication Date: 2015-06-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the kenaf varieties that are widely promoted in my country have small spines on the stems and petioles, and the whole calyx of the capsule is covered with dense white hairs, which brings great pain to the field operations and seed harvesting of hemp farmers.
In order to alleviate the pain of the skin in the production process of hemp farmers, as early as the 1940s, the Uzbek Experimental Station of the former Soviet Union carried out grafting and hybridization of kenaf and rose hemp in order to create a new thornless variety, but no high-yield thornless kenaf was selected. According to reports on the application of new varieties in production, Fujian Agriculture and Forestry University adopted mutagenesis technology and successively bred new thornless kenaf varieties Jinguang No. 1 and Jinguang No. 2 in 2005. However, the growth period of the above two varieties is short and the fiber yield is slightly low. There are certain limitations in production application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Using the purple flower maintainer line Fuzi 992 as the female parent and the basic vegetative maintainer line Fuhonghang 2B as the male parent, the first-generation hybrid F 1 generation;

[0021] (2) planting F 1 For the first generation, when flowering, it is used as the female parent, and the thornless maintainer Zanyin No. 1 is used as the male parent, and the hybrid generation is produced by hybridization;

[0022] (3) Plant the first generation of recrossing and harvest F 2 generation seeds,

[0023] (4) Planting isolated F under long-sunlight conditions in Fuzhou summer 2 2000 plants in the isolated population, in the mid-growth stage, manually touch the upper, middle, and lower parts of the stems of each individual kenaf in the isolated population, and pull out the single plant with thorns in the stem; and pull out the corolla again during the flowering stage Yellow single plant, select a single plant with purple corolla (genotype is AAcc), harvest F 3...

Embodiment 2

[0031] (1) The purple flower maintainer line "Fuzi 992" was used as the female parent, and the basic vegetative type maintainer line "Fuhonghang 2B" was used as the male parent to produce the first-generation hybrid F 1 generation;

[0032] (2) planting F 1 When it blooms, it is used as the female parent, and the thornless maintainer line "Jinguang 1" is used as the male parent, and the multicross generation is produced by hybridization;

[0033] (3) Plant the first generation of recrossing and harvest F 2 generation seeds,

[0034] (4) Planting isolated F under long-sunlight conditions in Fuzhou summer 2 1500 plants in the isolated population, in the mid-growth stage, manually touch the upper, middle, and lower parts of the stems of each individual kenaf in the isolated population, and pull out the single plant with thorns in the stem; and pull out the corolla again during the flowering stage Yellow single plant, select a single plant with purple corolla (genotype is AAcc...

Embodiment 3

[0042] (1) Using the purple flower maintainer line Fuzi 992 as the female parent and the basic vegetative maintainer line Fuhonghang 2B as the male parent, the first-generation hybrid F 1 generation;

[0043] (2) planting F 1 For the generation, when it blooms, it is used as the female parent, and the thornless germplasm resource Xin'an thornless is used as the male parent, and the hybrid generation is produced by hybridization;

[0044] (3) Plant the first generation of recrossing and harvest F 2 generation seeds,

[0045] (4) Planting isolated F under long-sunlight conditions in Fuzhou summer 2 2,500 plants were isolated from the population (Since Xin'an Wuthorn has a recovery effect on fertility, the population should be increased to determine the isolation of single plants with target traits). In the mid-growth period, the upper, middle and lower parts of the stems of each individual kenaf in the isolated population were manually touched plant by plant, and the individ...

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Abstract

The invention relates to a breeding method of a basic nutritional cytoplasmic male sterile line of stingless purple flowers of hibiscus cannabinus. The method comprises the following steps: carrying out test cross by taking an existing cytoplasmic male sterile line of the hibiscus cannabinus as a female parent and a stingless basic nutritional purple material of the hibiscus cannabinus as a male parent, selecting first generation of test cross plants as a sterile line; carrying out saturated backcross on a corresponding stingless basic nutritional purple material of the hibiscus cannabinus as a recurrent male parent; and finally breeding the basic nutritional cytoplasmic male sterile line of the stingless purple flowers of the hibiscus cannabinus, wherein the comprehensive characters are consistent with those of the male parent and represent cytoplasmic male sterility and stable sterility, and the recurrent male parent is a corresponding maintainer line.

Description

technical field [0001] The invention specifically relates to a method for selecting a thornless basic vegetative cytoplasmic male sterile line of kenaf, belonging to the field of crop genetics and breeding. Background technique [0002] Fujian Province is one of the three major jute kenaf breeding and seed retention bases in the country. It has good natural light and temperature conditions and is known for its excellent seed quality. However, kenaf is a frequent outcrossing crop, which is an insect-mediated flower, and the outcrossing rate can reach more than 30%. This kind of purification caused great difficulty. The purity of the kenaf male sterile line is the key to ensure the purity of the hybrid kenaf seed, so the breeding of the purple corolla male sterile line that is easy to identify and remove impurities in field production is of great significance to improve the purity of the hybrid kenaf seed. In addition, the kenaf varieties widely promoted in my country have t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 林荔辉祁建民吴建梅林培清徐建堂陶爱芬池仁漫方平平张立武林日新
Owner FUJIAN AGRI & FORESTRY UNIV
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