Breeding method of basic nutritional cytoplasmic male sterile line of stingless purple flowers of hibiscus cannabinus
A male sterile line, nutritional technology, applied in the field of crop genetics and breeding, can solve the problems of decreased biological yield and fiber yield, and achieve the effect of alleviating the pain of skin, ensuring production safety, and ensuring the purity of germplasm.
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Embodiment 1
[0020] (1) Using the purple flower maintainer line Fuzi 992 as the female parent and the basic vegetative maintainer line Fuhonghang 2B as the male parent, the first-generation hybrid F 1 generation;
[0021] (2) planting F 1 For the first generation, when flowering, it is used as the female parent, and the thornless maintainer Zanyin No. 1 is used as the male parent, and the hybrid generation is produced by hybridization;
[0022] (3) Plant the first generation of recrossing and harvest F 2 generation seeds,
[0023] (4) Planting isolated F under long-sunlight conditions in Fuzhou summer 2 2000 plants in the isolated population, in the mid-growth stage, manually touch the upper, middle, and lower parts of the stems of each individual kenaf in the isolated population, and pull out the single plant with thorns in the stem; and pull out the corolla again during the flowering stage Yellow single plant, select a single plant with purple corolla (genotype is AAcc), harvest F 3...
Embodiment 2
[0031] (1) The purple flower maintainer line "Fuzi 992" was used as the female parent, and the basic vegetative type maintainer line "Fuhonghang 2B" was used as the male parent to produce the first-generation hybrid F 1 generation;
[0032] (2) planting F 1 When it blooms, it is used as the female parent, and the thornless maintainer line "Jinguang 1" is used as the male parent, and the multicross generation is produced by hybridization;
[0033] (3) Plant the first generation of recrossing and harvest F 2 generation seeds,
[0034] (4) Planting isolated F under long-sunlight conditions in Fuzhou summer 2 1500 plants in the isolated population, in the mid-growth stage, manually touch the upper, middle, and lower parts of the stems of each individual kenaf in the isolated population, and pull out the single plant with thorns in the stem; and pull out the corolla again during the flowering stage Yellow single plant, select a single plant with purple corolla (genotype is AAcc...
Embodiment 3
[0042] (1) Using the purple flower maintainer line Fuzi 992 as the female parent and the basic vegetative maintainer line Fuhonghang 2B as the male parent, the first-generation hybrid F 1 generation;
[0043] (2) planting F 1 For the generation, when it blooms, it is used as the female parent, and the thornless germplasm resource Xin'an thornless is used as the male parent, and the hybrid generation is produced by hybridization;
[0044] (3) Plant the first generation of recrossing and harvest F 2 generation seeds,
[0045] (4) Planting isolated F under long-sunlight conditions in Fuzhou summer 2 2,500 plants were isolated from the population (Since Xin'an Wuthorn has a recovery effect on fertility, the population should be increased to determine the isolation of single plants with target traits). In the mid-growth period, the upper, middle and lower parts of the stems of each individual kenaf in the isolated population were manually touched plant by plant, and the individ...
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