Preparation method for platinum-base alloy-labeled three-channel sandwich type immunosensor and application
An immunosensor, platinum-based alloy technology, applied in the field of three-channel sandwich type immunosensor preparation, to achieve the effect of high catalytic performance, strong electrical activity and good biocompatibility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0029] Example 1 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker
[0030] (1) Grinding and polishing the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, and then washing with ultra-pure water to remove surface impurities and complete the pretreatment of the glassy carbon working electrode;
[0031] (2) Sonicate 3 μL, 1.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;
[0032] (3) Apply 3 μL, 8 μg / mL capture antibody of analyte a, capture antibody of analyte b, and capture antibody of analyte c onto the surface of the working electrode respectively, and dry in a refrigerator at 4 °C;
[0033] (4) Drop-coat 2 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place in a refrigerator at 4°C until dry, wash with ultrapure water, and dry in a refrigerator ...
Embodiment 2
[0036] Example 2 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker
[0037] (1) Grinding and polishing the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, and then washing with ultra-pure water to remove surface impurities and complete the pretreatment of the glassy carbon working electrode;
[0038] (2) Sonicate 4 μL, 2.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;
[0039] (3) Apply 4 μL and 10 μg / mL of the capture antibody for analyte a, capture antibody for analyte b, and capture antibody for analyte c to the surface of the working electrode respectively, and dry in a refrigerator at 4 °C;
[0040] (4) Drop-coat 3 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place it in a refrigerator at 4°C until dry, wash it with ultrapure water, and dry ...
Embodiment 3
[0043] Example 3 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker
[0044](1) Grinding and polishing the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, and then washing with ultra-pure water to remove surface impurities and complete the pretreatment of the glassy carbon working electrode;
[0045] (2) Sonicate 5 μL, 3.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;
[0046] (3) Apply 5 μL, 12 μg / mL capture antibody of analyte a, capture antibody of analyte b, and capture antibody of analyte c onto the surface of the working electrode respectively, and dry in a refrigerator at 4 °C;
[0047] (4) Drop-coat 4 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place in a refrigerator at 4°C until dry, wash with ultrapure water, and dry in a refrigerator ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com