Application of phosphoglycerate kinase 1 to preparation of rheumatoid arthritis diagnosis reagent

A diagnostic reagent, arthritis technology, applied in the field of application of phosphoglycerate kinase 1 in the preparation of rheumatoid arthritis diagnostic reagents, to achieve the effect of high specificity and strong sensitivity

Inactive Publication Date: 2015-06-10
常晓天
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this technology to determine the key enzymes of glucose metabolism and their functions, and to further understand the pathogenesis of RA, is th

Method used

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  • Application of phosphoglycerate kinase 1 to preparation of rheumatoid arthritis diagnosis reagent
  • Application of phosphoglycerate kinase 1 to preparation of rheumatoid arthritis diagnosis reagent
  • Application of phosphoglycerate kinase 1 to preparation of rheumatoid arthritis diagnosis reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Discovery and verification of PGK1 as a new blood marker for clinical diagnosis of rheumatoid arthritis

[0065] 1.1 Establishment of type II collagen-induced arthritis (CIA) rat model:

[0066] Male Wistar rats (n=20, each rat weighing 150-200 g) were obtained from the Animal Science and Health Science Experimental Center of Peking University (Beijing, China). These mice were randomly divided into two groups, CIA group and control group. Mice in all experimental groups were intradermally injected with a CFA solution containing 500 μg of bovine collagen II (SIGMA-ALDRICH, USA) at the base of their tails. And after 14 days, use the same method to inject again. In the same way, the control group was injected with BSA instead of type II collagen at the base of the tail. CIA rats were clinically scored according to the clinical manifestations of each carpal tarsal joint, and the evaluation criteria were as follows: 0=normal, 1=slight edema and erythema, 2=obvi...

Embodiment 2

[0096] Example 2: Diagnostic reagents and detection methods for clinical diagnosis of rheumatoid arthritis

[0097] Using PGK1 as a blood marker of rheumatoid arthritis, a diagnostic reagent for clinical diagnosis of rheumatoid arthritis is prepared, and blood of patients is detected. Blood samples were obtained by standard venipuncture from RA patients (n=120, 75 females, aged 20–75 years). Chronic joint inflammation for at least 3 years, erythrocyte sedimentation rate (ESR, 19 to 22 mm / hour, average 44 mm / hour), c-reactive protein (8-196 U / ml, average 29.1 U / ml) and rheumatoid factor (10.5- 2090U / ml) levels increased. Combined therapy with methotrexate and leflunomide for at least two months. 120 (80 females; 24-58 years old, average 40.2 years old) healthy blood donors. Blood samples were placed in EDTA-K2 vacuum blood collection tubes.

[0098] The composition of the diagnostic reagent includes: carbonate buffer solution, PBST washing solution, blocking solution, chrom...

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Abstract

The invention discloses a blood marker-PGK1 for rheumatoid arthritis clinical diagnosis, and discloses a reagent for rheumatoid arthritis clinical diagnosis. The reagent comprises a PGK1 antibody, an HRP-IgG antibody and a conventional reagent in a blood diagnosis reagent. The conventional reagent in the blood diagnosis reagent comprises a carbonate buffer solution, PBST washing liquor, confining liquid, a developing solution and a stop solution. The prepared detection reagent has the advantages of being high in sensitivity, high in specificity and the like and is capable of being widely applied to preliminary survey and health examination of clinical rheumatoid arthritis and providing a reliable basis for clinical diagnosis.

Description

technical field [0001] The invention relates to the application of phosphoglycerate kinase 1 (phosphoglycerate kinase 1, PKG1) in the preparation of diagnostic reagents for rheumatoid arthritis. Background technique [0002] Glucose metabolism mainly refers to the process of glucose degradation, including glycolysis, aerobic oxidation and pentose phosphate pathway metabolism. Glucose metabolism is one of the main metabolic pathways in the body. Glucose metabolism not only provides energy for physical activities, but also mediates a variety of physiological processes through complex signaling networks. Glucose metabolism plays an important role in many diseases such as diabetes, stroke, schizophrenia and drug abuse. [0003] Rheumatoid arthritis (RA) is an autoimmune disease. Recent studies have shown that glucose metabolism plays an important role in the pathogenesis of RA. Henderson. et al. found that the key enzymes of the glycolytic pathway, phosphoglyceraldehyde dehy...

Claims

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Application Information

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IPC IPC(8): G01N33/96
CPCG01N33/573G01N2800/102
Inventor 常晓天
Owner 常晓天
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