Lysostaphin compound enzyme disinfectant and preparation method thereof

A technology of lysostaphin and lysostaptococcus, which is applied in the directions of antibacterial drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems of being unsuitable for large-scale production and application, complicated preparation process, low disinfection efficiency, etc. The effect of healing, high activity, good disinfection effect

Inactive Publication Date: 2015-06-24
杨陈
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, disinfectants are mainly chemical disinfectants such as antibiotics and bacteriostats. These disinfectants mainly achieve the purpose of disinfection through protein denaturation or microbial dissolution. Although these chemical disinfectants can achieve the purpose of disinfection, their The preparation process is relatively complicated, and it will pollute the environment during the production process, so it is not suitable for large-scale production and application
Moreover, chemical disinfectants also have the disadvantages of low disinfection efficiency, toxic side effects, and difficult preservation in clinical application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lysostaphin compound enzyme disinfectant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A lysostaphin composite enzyme disinfectant, comprising the following components in parts by mass: 6 parts of lysostaphin, 6 parts of antibacterial peptides, 6 parts of chlorhexidine acetate, 13 parts of sorbitol, 5 parts of sodium dihydrogen phosphate and 3 parts of disodium hydrogen phosphate.

[0028] The preparation method of above-mentioned lysostaphin composite enzyme disinfectant comprises the steps:

[0029] 1) Dissolve 6 parts of lysostaphin, 6 parts of antimicrobial peptide and 6 parts of chlorhexidine acetate in distilled water and mix well;

[0030] 2) Add 13 parts of sorbitol to the solution obtained in step 1), and shake well;

[0031] 3) Dissolve 5 parts of sodium dihydrogen phosphate and 3 parts of disodium hydrogen phosphate in distilled water and stir well, then add the obtained solution to the solution obtained in step 2), and shake well to obtain 1# disinfectant.

Embodiment 2

[0033] A lysostaphin composite enzyme disinfectant, comprising the following components in parts by mass: 8 parts of lysostaphin, 3 parts of antibacterial peptides, 3 parts of chlorhexidine acetate, 16 parts of sorbitol, 1.1 parts of sodium dihydrogen phosphate and 10 parts of disodium hydrogen phosphate.

[0034] The preparation method of above-mentioned lysostaphin composite enzyme disinfectant comprises the steps:

[0035] 1) Dissolve 8 parts of lysostaphin, 3 parts of antimicrobial peptide and 3 parts of chlorhexidine acetate in distilled water and mix well;

[0036] 2) Add 16 parts of sorbitol to the solution obtained in step 1), and shake well;

[0037] 3) Dissolve 1.1 parts of sodium dihydrogen phosphate and 10 parts of disodium hydrogen phosphate in distilled water and stir well, then add the obtained solution to the solution obtained in step 2), and shake well to obtain 2# disinfectant.

Embodiment 3

[0039] A lysostaphin composite enzyme disinfectant, comprising the following components in parts by mass: 2 parts of lysostaphin, 7 parts of antibacterial peptides, 9 parts of chlorhexidine acetate, 19 parts of sorbitol, 10 parts of sodium dihydrogen phosphate, Disodium hydrogen phosphate 1.1 parts.

[0040] The preparation method of above-mentioned lysostaphin composite enzyme disinfectant comprises the steps:

[0041] 1) Dissolve 2 parts of lysostaphin, 7 parts of antimicrobial peptide and 9 parts of chlorhexidine acetate in distilled water and mix well;

[0042] 2) Add 19 parts of sorbitol to the solution obtained in step 1), and shake well;

[0043] 3) Mix 10 parts of sodium dihydrogen phosphate and 1.1 parts of disodium hydrogen phosphate and stir well, then add the mixture of the two to the mixture obtained in step 2), shake well to get 3# disinfectant.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a lysostaphin compound enzyme disinfectant comprising the following components in parts by weight: 1.1-10 parts of lysostaphin, 1.1-10 parts of antimicrobial peptide, 1.1-10 parts of chlorhexidine acetate, 10-20 parts of sorbitol, 1.1-10 parts of sodium dihydrogen phosphate and 1.1-10 parts of disodium hydrogen phosphate. The lysostaphin compound enzyme disinfectant has the advantages of high disinfection efficiency, good disinfection effect, easy storage and stable performance. The invention further discloses a preparation method of the lysostaphin compound enzyme disinfectant. The preparation method mainly comprises the following steps: 1) dissolving the lysostaphin, the antimicrobial peptide and the chlorhexidine acetate in water, mixing the solutions, and shaking uniformly; 2) adding sorbitol into the solution obtained in the step 1), and shaking uniformly; and 3) dissolving the sodium dihydrogen phosphate and the disodium hydrogen phosphate in distilled water, stirring uniformly, adding the obtained solution into the solution obtained in the step 2), and shaking uniformly to obtain the lysostaphin compound enzyme disinfectant. The preparation method has the advantages of simple process and strong operability.

Description

technical field [0001] The invention belongs to the field of biological preparations, and in particular relates to a lysostaphin composite disinfectant and a preparation method thereof. Background technique [0002] Disinfectants mainly refer to preparations used to kill pathogenic microorganisms on the transmission medium to make them harmless. At present, disinfectants are mainly chemical disinfectants such as antibiotics and bacteriostats. These disinfectants mainly achieve the purpose of disinfection through protein denaturation or microbial dissolution. Although these chemical disinfectants can achieve the purpose of disinfection, their The preparation process is relatively complicated, and it will pollute the environment during the production process, so it is not suitable for large-scale production and application. Moreover, chemical disinfectants also have the disadvantages of low disinfection efficiency, toxic and side effects, and difficult preservation in clinica...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/47A61K47/26A61K47/02A61P31/04A61P17/02A61K38/00A61K31/155
Inventor 杨陈
Owner 杨陈
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap