Supercharge Your Innovation With Domain-Expert AI Agents!

Starch endosperm-specific non-expression promoter nse and its application

A technology of DNA molecules in the sequence listing, applied in the fields of application, recombinant DNA technology, DNA/RNA fragments, etc., it can solve problems such as differences in expression levels of expression profiles, different expression effects, and difficulty in meeting the actual needs of plant genetic engineering, and achieve improved The effect of receiving, saving energy and improving seed quality

Active Publication Date: 2017-06-20
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These patents provide a favorable promoter tool for the application of transgenic plants, but because different patents involve different promoters, there are obvious differences in their expression profiles (that is, specific expression sites and expression levels); and the same promoter is used in different Monocotyledonous and dicotyledonous plants, even between different species often have different expression effects
Therefore, the existing promoter resources are difficult to meet the actual needs of plant genetic engineering

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Starch endosperm-specific non-expression promoter nse and its application
  • Starch endosperm-specific non-expression promoter nse and its application
  • Starch endosperm-specific non-expression promoter nse and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the acquisition of rice NSE promoter

[0030] From rice 'Nipponbare' (Oryza sativa ssp.japonicacultivarNipponbare) by CTAB method, described in Yuman Zhang, Yongsheng Yan, Lina Wang, Kun Yang, NaXiao, Yunfeng Liu, Yaping Fu, Zongxiu Sun, Rongxiang Fang, Xiaoying Chen. A novelrice gene, NRR responds to macronutrient deficiency and regulates root growth.Mol.Plant, 2012,5(1):63-72. The public can obtain it from the Institute of Microbiology, Chinese Academy of Sciences.) Genomic DNA was extracted from the leaves, and forward and reverse specific primers NSE were designed -S-Nsi: 5'-ATGCATGCCCTGGCTAGCTTCTTCATCACTTC-3' (sequence 2); NSE-R-BH: 5'-GGATCCGGCCTGCACCGCAAATGGCGGATTTCC-3' (sequence 3) (underlined bases are NsiI and BamHI restriction enzymes introduced respectively cleavage site), using TaKaRa LA-Taq DNA polymerase for PCR amplification to obtain a 2,208bp PCR product. The PCR product was sent for sequencing. As a result, the DNA molecule represented ...

Embodiment 2

[0031] Example 2, Functional Verification of Rice NSE Promoter

[0032] 1. Obtaining the recombinant vector

[0033] The PCR product obtained in Example 1 was digested with NsiI and BamHI to obtain a 2,208bp digested product, which was combined with the plant expression vector pCAMBIA-1300 digested with PstI (the same tail enzyme as NsiI) and BamHI -SGN (recorded in Yuman Zhang, Yongsheng Yan, Lina Wang, Kun Yang, Na Xiao, Yunfeng Liu, Yaping Fu, Zongxiu Sun, Rongxiang Fang, Xiaoying Chen. A novel rice gene, NRR responds tomato nutrient deficiency and regulates root growth. Mol. Plant, 2012,5(1):63-72. The public can obtain it from the Institute of Microbiology, Chinese Academy of Sciences. The promoter driving the expression of the GUS gene in this vector is 35S) linked to obtain a recombinant vector.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a starch endosperm-specific non-expression promoter NSE and its application. The present invention provides a DNA fragment, which is the following 1) or 2) or 3) DNA molecule: 1) the DNA molecule shown in sequence 1 in the sequence listing; 2) hybridizes with 1) the defined DNA sequence under stringent conditions 3) A DNA molecule that has more than 90% homology with the DNA sequence defined in 1) and has a promoter function. Experiments of the present invention have proved that the present invention clones the promoter NSE, and the NSE promoter drives the high-efficiency expression of the target gene only in parts other than the starch endosperm, and it is applied to crops with the starch endosperm as the main harvest product, and the exogenous gene ( Such as herbicide resistance, resistance to diseases and insect pests, etc.) can be selectively expressed, which can not only save energy, but also improve the public's acceptance of genetically modified food.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a starch endosperm-specific non-expression promoter NSE and its application. Background technique [0002] In plant genetic engineering, for plants with seeds and fruits as the main harvest products, the use of seed non-expression promoters to drive the target gene to be expressed only in green tissues such as stems and leaves, but not in edible parts of seeds, is an important way to improve the quality of genetically modified foods. An effective strategy for security. [0003] At present, domestic and foreign patents related to non-expression in plant seeds are as follows: (1) Application number: 201210202831.8: a green tissue-specific promoter for citrus; (2) Application number: 200710051443.3: Tissue-specific expression promoter PD540 and its expression in rice Application in improvement; (3) Application number: 01823070.9: vegetative growth-specific promoter and genetically reco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/84C12N1/21C12N5/10C12N15/11
Inventor 方荣祥陈晓英张玉满
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com