Rabbit kidney in-vivo low-temperature mechanical perfusion model and construction method thereof

Abstract

The invention belongs to the technical field of medical animal models, particularly to a rabbit kidney in-vivo low-temperature mechanical perfusion model and a construction method thereof. The rabbit kidney in-vivo low-temperature mechanical perfusion model which serves as a research tool is characterized in that the in-vivo autologous kidney transplant mode is adopted, wherein the kidney transplant low-temperature mechanical perfusion process is copied. The construction method comprises the steps of preoperative preparation; anesthesia, fixation, skin preparation and conventional laparotomy; renal pedicle clipping and abdominal exploration; reperfusion; in-vivo simulation on kidney in-vitro low-temperature MP (Mechanical Perfusion) preservation; vascular suture; bladder colostomy; right kidney excision; abdominal cavity closing; postoperative maintenance. According to the rabbit kidney in-vivo low-temperature mechanical perfusion model, the immunological rejection caused by allogeneic kidney supply is eliminated; meanwhile the kidney is not completely separated and accordingly the vascular suture is easy, the suture time is shortened, the vascular damage is reduced, and the interference of the factors is reduced when the rabbit kidney in-vivo low-temperature mechanical perfusion model is applied to the MP mechanism research; the operation is simple and the repeatability is good.

Description

technical field [0001] The invention belongs to the technical field of medical animal models, and in particular relates to a method for constructing a rabbit kidney in vivo low-temperature mechanical perfusion model. Background technique [0002] Kidney transplantation is the most effective treatment for end-stage renal disease. According to statistics, China currently performs about 5,000 cases of kidney transplantation every year [1], but the increasingly serious shortage of donor kidneys plagues the majority of patients and transplant doctors. For this reason, the Ministry of Health has started the pilot work of donation after cardiac death (DCD) since 2010, and it has been running well. Since January 1, 2015, organ donation after the death of citizens has become the only source of donors . However, compared with donor after brain death (DBD), DCD has some disadvantages [2]. For example, the warm ischemia time of DCD donor organs is longer, resulting in a high inciden...

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Problems solved by technology

Hauet T et al. published an article about the model making method of pig autologous kidney transplantation in J Am Soc Nephrol in 2000. The immune rejection caused by the kidney can eliminate the interference of the immune system, but there are defects such as long suturing time and large vascular damage, which still affect the observation of the protective mechanism of MP [11]

In addition, referring to the whole process of clinical kidney transplantation, is it possible to simulate the whole process of human DCD kidney transplantation on small animals? Rabbits, compared with mice, are less difficult to experiment with. Although Gregory M. Fahy and Suja E. Ali have done normal temperature perfusion of rabbit kidneys [12], they have not used rabbits as a model to study low temperature mechanical perfusion. mechanism reporting

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