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Thiamphenicol nanoliposome and preparation method thereof

A nanoliposome, thiamphenicol technology, applied in the directions of liposome delivery, antibacterial drugs, etc., can solve the problem of dosage form limitation, poor water solubility of thiamphenicol, etc., achieve simple operation, improve the immunity of livestock and poultry Effect

Inactive Publication Date: 2015-07-01
HENAN HUITONG TIANXIA BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor water solubility of thiamphenicol, the dosage form is limited
The reports are mainly powders and tablets, and there are few reports about thiamphenicol nanoliposomes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The prescription of thiamphenicol nanoliposome is: thiamphenicol 0.8 g, lecithin 3.0 g, cholesterol 0.6 g, Tween-80 3.0 g, vitamin C 0.3 g.

[0017] The preparation method of thiamphenicol nano liposome is:

[0018] (1) Weigh the prescribed amount of thiamphenicol, lecithin, cholesterol, Tween-80 and vitamin C into a round bottom flask, add chloroform-methanol solvent to fully dissolve it, wherein the chloroform-methanol solvent is made of chloroform and Methanol is prepared by mixing with a volume ratio of 1:1;

[0019] (2) Add 100 mL of pH 7.4 phosphate buffer, and sonicate in a water bath to make it into a milky liquid.

[0020] (3) Under the condition of water bath at 40 ℃, remove the organic solvent by rotary evaporation until gel is formed, and continue to evaporate under reduced pressure until a suspension is formed.

[0021] (4) Continue to evaporate to further remove the organic solvent, fill with N 2 Until the smell of chloroform-methanol disappears, put it...

Embodiment 2

[0023] The prescription of thiamphenicol nanoliposome is: thiamphenicol 1.0 g, lecithin 5.0 g, cholesterol 1.0 g, Tween-80 3.0 g, vitamin C 0.3 g.

[0024] The preparation method of thiamphenicol nano liposome is:

[0025] (1) Weigh the prescribed amount of thiamphenicol, lecithin, cholesterol, Tween-80 and vitamin C into a round bottom flask, add an appropriate amount of chloroform-methanol solvent to fully dissolve it, and the chloroform-methanol solvent is made of chloroform It is prepared by mixing with methanol at a volume ratio of 1:1.

[0026] (2) Add 100 mL of pH 7.4 phosphate buffer, and sonicate in a water bath to make it into a milky liquid.

[0027] (3) Under the condition of water bath at 40 ℃, remove the organic solvent by rotary evaporation until gel is formed, and continue to evaporate under reduced pressure until a suspension is formed.

[0028] (4) Continue to evaporate to further remove the organic solvent, fill with N 2 Until the smell of chloroform-meth...

Embodiment 3

[0030] The prescription of thiamphenicol nanoliposome is: thiamphenicol 0.8 g, lecithin 4.0 g, cholesterol 1.0 g, Tween-80 3.0 g, vitamin C 0.3 g.

[0031] The preparation method of thiamphenicol nano liposome is:

[0032] (1) Weigh the prescribed amount of thiamphenicol, lecithin, cholesterol, Tween-80 and vitamin C into a round bottom flask, add an appropriate amount of chloroform-methanol solvent to fully dissolve it, and the chloroform-methanol solvent is made of chloroform It is prepared by mixing with methanol at a volume ratio of 1:1.

[0033] (2) Add 100 mL of pH 7.4 phosphate buffer, and sonicate in a water bath to make it into a milky liquid.

[0034] (3) Under the condition of water bath at 40 ℃, remove the organic solvent by rotary evaporation until gel is formed, and continue to evaporate under reduced pressure until a suspension is formed.

[0035] (4) Continue to evaporate to further remove the organic solvent, fill with N 2 Until the smell of chloroform-meth...

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Abstract

The invention discloses a thiamphenicol nanoliposome and a preparation method thereof. The thiamphenicol nanoliposome is prepared from thiamphenicol, phosphatidylcholine, cholesterol, vitamin C and Tween-80 in a ratio of 0.8-1.0: 3.0-5.0: 0.6-1.0: 0.3: 3.0. The preparation method comprises the following steps: placing the five raw materials in a round-bottom flask, adding a proper amount of chloroform-methanol solvent to fully dissolve the five raw materials, adding a phosphate buffer with a pH value of 7.4 and carrying out water-bath ultrasonic treatment so as to obtain a milk-like liquid; subjecting the milk-like liquid to rotary evaporation in a water bath with a temperature of 40 DEG C to remove the organic solvent until gel forms; subsequently carrying out pressure-reduced evaporation until a suspension liquid forms; continuing evaporation to further remove the organic solvent and introducing N2 until the smell of chloroform-methanol disappears; placing the obtained substance in a cryogenic refrigerator for refrigeration; taking the substance out for thawing at room temperature; and successively carrying out thawing, oscillation, freeze thawing, ultrasonic treatment, introduction of N2 and sealing, and preservation in a refrigerator with a temperature of 4 DEG C. The thiamphenicol nanoliposome can effectively treat diseases of livestock and poultry caused by bacterial infection and can enhance the immunity of livestock and poultry.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicine, and in particular relates to a thiamphenicol nanoliposome and a preparation method thereof. Background technique [0002] Thiamphenicol, also known as Thiamphenicol, is a broad-spectrum antibiotic of amidoalcohols. Its chemical name is 1-p-methylsulfonyl-phenyl-2-dichloroacetamide-1,3-propanediol. Its structure is similar to that of chloramphenicol. . It is mainly used to treat bacterial infections of livestock and poultry, especially respiratory and intestinal diseases caused by Escherichia coli, Salmonella and Pasteurella, and also used for septicemia, enteritis, Erythroderma and other bacterial diseases. However, due to the poor water solubility of thiamphenicol, the dosage form is limited. The reports are mainly powders and tablets, and there are few reports about thiamphenicol nanoliposomes. [0003] As a new type of pharmaceutical preparation, liposome has its unique advanta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/165A61P31/04
Inventor 张要齐李引乾孙雪峰杨亚军
Owner HENAN HUITONG TIANXIA BIO ENG