Anti-fatigue composition and preparation method thereof
A composition and anti-fatigue technology, applied in the fields of food preparation, food science, application, etc., can solve the problems of unstable function and ineffective anti-fatigue health care products
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Embodiment 1
[0093] The preparation of embodiment 1 apple stem cell freeze-dried powder
[0094] 1. The formula of the culture medium:
[0095] 1.1 Induction medium: MS solid medium + NAA0.5mg / L + BA 2mg / L + CH 1mg / L, pH value is 6.0;
[0096] 1.2 Proliferation medium: MS liquid medium+2,4-D 2.0mg / L+BA 1.0mg / L+CH1.0mg / L+AC 1.0mg / L, pH value is 6.0;
[0097] 2. Sterilization of apple branches
[0098] Choose green-planted apple trees free of chemical fertilizer and pesticide pollution, and select 10 10cm-long new shoots according to certain criteria; wash the surface attachments on the new shoots with 2L of sterile water; soak the new shoots in 120mg / L L-ascorbic acid 1 minute; sterilize new shoots in 75% ethanol solution for 1 minute, rinse with 2L sterile water; put them in 30% hydrogen peroxide solution for sterilization for 20 minutes, and rinse with sterile water for 10 minutes.
[0099] 3. Induction of cambium tissue
[0100] Use an inoculation knife to peel off the cambium, phloe...
Embodiment 2
[0109] The preparation of embodiment 2 anti-fatigue composition
[0110] First, 100 g of apple stem cell freeze-dried powder, 100 g of American ginseng extract, and 6 g of taurine are respectively pulverized, passed through a 120-mesh sieve, and then the pulverized and sieved materials are mixed evenly in a mixer.
Embodiment 3
[0111] The preparation of embodiment 3 anti-fatigue compositions
[0112] First, 100 g of apple stem cell freeze-dried powder, 100 g of American ginseng extract, and 15 g of taurine are respectively pulverized, passed through a 120-mesh sieve, and then the pulverized and sieved materials are mixed evenly in a mixer.
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