Fluorescent RT-PCR detection reagent of nipah virus M gene as well as preparation method and application of fluorescent RT-PCR detection reagent

A technology of RT-PCR, Nipah virus, applied in the field of biology
CN104774972AInactive Publication Date: 2015-07-15ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2015-07-15
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a fluorescent RT-PCR detection reagent of a nipah virus M gene as well as a preparation method and an application of the fluorescent RT-PCR detection reagent. A set of specific primers, a Taqman probe and a positive control are designed and synthesized, and the primers and the probe are used for establishing a fluorescent RT-PCR detection system which is quick, simple and convenient and is strong in specificity and high in sensitivity, so that the nipah virus M gene can be detected quickly, accurately, specifically, safely, simply and conveniently from a detected sample in 3-4 hours, and the fluorescent RT-PCR detection reagent can be used for detecting micro nipah virus M gene from domestic pigs and in related samples.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a fluorescent RT-PCR detection reagent for Nipah virus M gene and a preparation and use method thereof. Background technique

[0002] Nipah virus (Nipah Virus, NiV) is a new virus identified in 1999 that seriously endangers domestic pigs and humans, and can cause nervous system and respiratory diseases in humans and pigs (Chua, K.B., Bellini, W.J., Rota, P.A. , 2000. Nipah virus: a recently emergent deadly paramyxovirus. Science 288, 1432–1435.). In terms of classification, Nipah virus is a member of the Henipavirus genus of the subfamily Paramyxoviridae. Its genome is a single-stranded negative-sense RNA with a total length of about 18.2 kb. It contains 6 transcription units, which encode nucleoprotein (N), phosphorus Six structural proteins including protein (P), matrix protein (M), fusion protein (F), receptor binding protein (G) and large protein (L) ( Harcourt B, T...

Claims

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