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A method for inducing adventitious buds by using fibrous roots of rubber grass

A technology of rubber grass and fibrous roots is applied in the field of plant cell engineering, which can solve the problems of high cost and complicated operation process, and achieve the effects of low cost, simple induction medium and simple formula.

Active Publication Date: 2018-01-05
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing rubber grass tissue culture techniques all have the disadvantages of cumbersome operation process, need to transfer different media for induction and subculture, and high cost

Method used

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  • A method for inducing adventitious buds by using fibrous roots of rubber grass
  • A method for inducing adventitious buds by using fibrous roots of rubber grass

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The present embodiment relates to a method for inducing adventitious buds by fibrous roots of rubber grass, and the specific steps are as follows:

[0030] 1) Preparation and disinfection of medium: prepare 1L MS medium, add 20g / L sucrose, 7g / L agar powder, 1.5mg / L 6-BA and 0.4mg / L IAA to it. After configuration, adjust the pH to 6.0-7.0 with 1.0mol / L NaOH and 1.0mol / L HCl solution, sterilize at 121±2°C and steam pressure of 103.4kPa for 15 minutes, and store the tissue culture bottle at 121±2°C , sterilized under the condition of steam pressure 103.4kPa for 30 minutes, the sterilized culture medium was subpackaged in the ultra-clean workbench, each bottle was about 30mL, and it was used for inoculation after solidification.

[0031] 2) Inoculation and induction of explants: select fibrous roots of aseptic seedlings of rubber grass, cut into 1-2cm long root segments and inoculate them in induction medium for adventitious bud induction, 16 hours of light per day, light i...

Embodiment 2

[0033] The present embodiment relates to a method for inducing adventitious buds by fibrous roots of rubber grass, and the specific steps are as follows:

[0034] 1) Preparation and disinfection of medium: Prepare 1L MS medium, add 25g / L sucrose, 8g / L agar powder, 0.5mg / L 6-BA and 0.2mg / L IAA to it. After configuration, adjust the pH to 6.0-7.0 with 1.0mol / L NaOH and 1.0mol / L HCl solution, sterilize at 121±2°C and steam pressure of 103.4kPa for 15 minutes, and store the tissue culture bottle at 121±2°C , sterilized under the condition of steam pressure 103.4kPa for 30 minutes, the sterilized culture medium was subpackaged in the ultra-clean workbench, each bottle was about 30mL, and it was used for inoculation after solidification.

[0035] 2) Inoculation and induction of explants: select the fibrous roots of aseptic seedlings of rubber grass, cut into 1-2cm long root segments and inoculate them in the induction medium for adventitious bud induction, light for 14 hours a day, ...

Embodiment 3

[0037] The present embodiment relates to a method for inducing adventitious buds by fibrous roots of rubber grass, and the specific steps are as follows:

[0038]1) Preparation and disinfection of medium: prepare 1L MS medium, add 30g / L sucrose, 8g / L agar powder, 1mg / L 6-BA and 0.5mg / L IAA to it. After configuration, adjust the pH to 6.0-7.0 with 1.0mol / L NaOH and 1.0mol / L HCl solution, and sterilize for 15 minutes at 121±2°C and steam pressure of 103.4kPa. ℃, steam pressure of 103.4kPa for 30 minutes, the sterilized culture medium is divided in the ultra-clean workbench, each bottle is about 30mL, and it is used for inoculation after solidification.

[0039] 2) Inoculation and induction of explants: select the fibrous roots of aseptic seedlings of rubber grass, cut into 1-2cm long root segments and inoculate them on the induction medium for adventitious bud induction, light for 18 hours a day, light intensity 3200lux, temperature 22 -25°C, the inoculated roots turn green and...

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Abstract

The invention relates to a method for inducing adventitious buds by fibrous roots of rubber grass, comprising the following steps: 1) preparation and disinfection of culture medium: said culture medium is MS medium; and adding a final concentration of 20 ~30g / L of sucrose, 7~8g / L of agar powder, 0.5~1.5mg / L of 6‑BA and 0.2~0.5mg / L of IAA; 2) inoculation and induction of adventitious buds: aseptically The fibrous roots of the seedlings are inoculated in the culture medium for adventitious bud induction, and the light is 14-18 hours a day, the light intensity is 2800-3200 lux, and the temperature is 22‑25 °C. The method of the invention has the advantages of simple medium formula, low cost, simple operation, high induction rate and good seedling quality.

Description

technical field [0001] The invention belongs to the field of plant cell engineering and relates to a method for efficiently inducing adventitious buds by using fibrous roots of rubber grass as explants. Background technique [0002] Rubber grass, also known as Russian dandelion, has high rubber content in its roots, excellent quality, strong adaptability, short growth and harvest period, suitable for mechanized planting and harvesting, strong stress resistance, good biocompatibility, and strong anti-allergic performance. It is an important rubber-producing crop that has attracted much attention in recent years. Rubber grass is self-incompatible, has a low seed setting rate, and a high hybrid rate. Propagation by seeds is not conducive to the preservation and reproduction of fine varieties. Moreover, the seed reproduction cycle is long and the reproduction speed is slow, which is difficult to meet the requirements of commercial production. Therefore, the research and develop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 覃碧张立群王锋刘实忠高玉尧杨玉双甘霖徐建欣
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI