Preparation and application method of molecular imprinting electrochemical sensor

A technology of molecular imprinting and application method, applied in the field of chemical detection, can solve the problems of inconvenient portability, complicated pretreatment, high price, etc., and achieve the effects of easy promotion, wide linear range and low detection limit.

Inactive Publication Date: 2015-07-29
BINZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection methods of β-agonists in food mainly include liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry and enzyme-linked immunoassay. These methods have the disadvantages of complicated pretreatment, high price and inconvenient portability

Method used

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  • Preparation and application method of molecular imprinting electrochemical sensor
  • Preparation and application method of molecular imprinting electrochemical sensor
  • Preparation and application method of molecular imprinting electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0056] Weigh (1+0.0050 g) actual samples (mutton, chicken, pork liver and pork) into 10 mL sample tubes, add different amounts of SAL standard solution and 3 mL of acetonitrile-acetone extract (V / V=4:1 ), after multiple times of ultrasonic centrifugation, the upper layer solution was transferred to a nitrogen blowpipe, concentrated and evaporated, and then transferred to 1.0 mL of PBS buffer solution with pH=7.0 for DPV detection, as shown in the table below. The recoveries of mutton, chicken, pork liver and pork were 86.5%-111.9% (n=3), and the standard deviations were 2.5%-4.3%. The results show that the sensor can be used for the detection of SAL in real samples.

[0057]

[0058] The sensor produced by the invention has good sensitivity and selectivity, and the detection of actual samples has been successfully carried out, and the determination recovery rate is 86.5%-111.9% (n=3), and the practicability is strong.

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Abstract

The invention discloses a preparation and application method of a molecular imprinting electrochemical sensor. The method comprises the following steps: selecting a glassy carbon electrode as a working electrode; performing surface pretreatment on the glassy carbon electrode through a cyclic voltammetry; modifying the pretreated glassy carbon electrode with nanogold, polythionine, methacrylic acid and a salbutamol composite membrane in sequence through electrochemical polymerization; finally eluting salbutamol, so as to obtain the molecular imprinting electrochemical sensor. According to the invention, salbutamol in food is detected through a differential pulse amperometric detection method, so that an application process is accomplished. Compared with the prior art, the preparation and application method has the advantages that the response speed is high, the sensitivity is high, the recognition performance is good, a wider linearity range and a lower detection limit are provided for rapidly detecting salbutamol in food, the practicability is high, and the method is easy for popularization.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a preparation and application method of a molecularly imprinted electrochemical sensor with strong practicability. Background technique [0002] Salbutamol (Salbutamol, SAL) is a β-type animal growth stimulant, chemical name: 1-(4-hydroxy-3-hydroxymethylphenyl)-2-(tert-butylamino)ethanol. SAL can promote the growth of animal skeletal muscle, accelerate the growth rate of animals, reduce fat accumulation, and is often added to livestock and poultry feed (also known as clenbuterol). The residual SAL in animals enters the human body through the food chain and accumulates in the body, causing side effects such as heart palpitations, headaches, muscle pain and trembling, and fever. China and many countries in the European Union have banned the use of SAL. Therefore, it is very important to establish a sensitive and rapid SAL detection method. [0003] At present, the det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 杜平
Owner BINZHOU UNIV
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