A method for detecting endogenous hormones in corn radicle sheaths

Abstract

The invention relates to the field of seed science, especially to a corn coleorhiza endogenous hormone detection technology based on a GC-MS internal standard method. The technology comprises processes of sample acquisition work, sample extraction work, purification and enrichment work, GC-MS / SIM detection work, post-processing and the like. A coleorhiza tissue sample is acquired by a horizontal and vertical stripping method; 80% methanol (cold) is used for extraction, and endogenous hormone is extracted through eight steps; by the use of a Sep-Pak C18 column, sample purification and enrichment including nine steps are carried out; hormone is detected by the use of GC-MS Agilent DB-17ms; and hormone content is obtained through post-processing such as optimization of detection conditions and the like. Contents of eight endogenous hormones of corn coleorhiza are detected based on the GC-MS internal standard method, and the detection effect is enhanced by the technical combination of ''horizontal and vertical stripping+ extraction+ solid-phase extraction+ Agilent DB-17ms''. The technology can be applied in researches on the mechanism of corn seed germination.

Description

technical field [0001] The invention relates to the field of seed science, in particular to a method for detecting endogenous hormones in corn radicle sheaths. Background technique [0002] Seed germination is the growth and development stage in which the embryo recovers from a relatively static state of life activities to a vigorous physiological metabolism. The process of seed germination is accompanied by various physiological and biochemical changes, such as: cell activation and repair, embryo growth and anabolism, etc. These physiological and biochemical changes are inseparable from the participation of plant endogenous hormones. Plant endogenous hormones are trace organic substances synthesized in plants that have a significant effect on plant growth and development. They can be transferred from the site of production to the site of action, and can regulate plant growth and development at low concentrations. They are almost involved in every process of seed germinatio...

AI Technical Summary

Problems solved by technology

However, the content of plant endogenous hormones is extremely low, their properties are unstable, and they are easily interfered by other secondary metabolites. In addition, the radicle sheath tissue is very small, and the amount of collection is very small, and there are differences in the extraction methods of endogenous hormones in different plant samples. Exploring and optimizing the extraction, purification, and detection methods and processes of maize radicle endogenous hormones, and improving the detection accuracy and effect of radicle endogenous hormones are critical to the study of maize seed germination mechanisms and other aspects

Traditional plant endogenous hormone extraction and detection methods have problems such as large sample requirements, easy to be affected by the environment, large detection errors, low detection sensitivity, and large solvent consumption.

Unable to meet the technical requirements for ultramicro quantitative and qualitative detection of endogenous hormones in the radicle sheath in the study of maize seed germination mechanism

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