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Rhodococcus ruber and application thereof in preparing decabromodiphenyl oxide degrading agent

A technology of decabromodiphenyl ether and rhodococcus, applied in rhodococcus and its application in the preparation of decabromodiphenyl ether degradation agent, can solve the problems of weak function and low efficiency of decabromodiphenyl ether

Active Publication Date: 2015-08-12
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported bacterial strains capable of decabromodiphenyl ether degradation include White rot fungi, Rhodococcus jostii RHA1, Burkholderia xenovorans LB400, Lysinibacillus fusiformis DB-1, etc., but their degradation efficiency of decabromodiphenyl ether is low and their functions are not strong

Method used

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  • Rhodococcus ruber and application thereof in preparing decabromodiphenyl oxide degrading agent
  • Rhodococcus ruber and application thereof in preparing decabromodiphenyl oxide degrading agent
  • Rhodococcus ruber and application thereof in preparing decabromodiphenyl oxide degrading agent

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Embodiment 1

[0023] 1. Enrichment, isolation and identification of Rhodococcus ruber TAW-CT127

[0024] 1. Enrichment, isolation and purification of Rhodococcus ruber TAW-CT127

[0025] Medium:

[0026] Artificial Seawater Medium (MMC): Each liter of medium contains 24g of NaCl, NH 4 NO 3 1g, KCl 0.7g, KH 2 PO 4 2.0g, Na 2 HPO 4 3.0g, pH 7.4, MgSO 4 7g (added after sterilization).

[0027] Trace element salt solution: every liter of trace element salt solution contains 2mg of CaCl, FeCl 3 ·6H 2 O 50mg, CuSO 4 0.5mg, MnCl 2 ·.H 2 O 0.5mg, ZnSO 4 ·.7H 2 O 10 mg.

[0028] Marine bacteria agar 2216 medium and marine bacteria broth 2216 medium were purchased from BD Medical Instruments (Shanghai) Co., Ltd.

[0029] Decabromodiphenyl ether mother liquor: organic solvent dichloromethane (chromatographic grade), decabromodiphenyl ether concentration 1g / L

[0030] The Rhodococcus ruber (Rhodococcus ruber) TAW-CT127 of the present invention is obtained through enrichment, separation...

Embodiment 2

[0042] Degradation Analysis of Decabromodiphenyl Ether by Rhodococcus ruber TAW-CT127

[0043] Artificial Seawater Medium (MMC): Each liter of medium contains 24g of NaCl, NH 4 NO 3 1g, KCl 0.7g, KH 2 PO 4 2.0g, Na 2 HPO 4 3.0g, pH 7.4, MgSO 4 7g (added after sterilization).

[0044] Trace element salt solution: every liter of trace element salt solution contains 2mg of CaCl, FeCl 3 ·6H 2 O 50mg, CuSO 4 0.5mg, MnCl 2 ·.H 2 O 0.5mg, ZnSO 4 ·.7H 2 O 10 mg.

[0045] Enrichment Medium EM (Enrichment Medium): Marine Bacteria Broth 2216 Medium

[0046] Decabromodiphenyl ether degradation medium: 100mL MMC medium + trace elements

[0047] Proliferation culture of Rhodococcus ruber (Rhodococcus ruber) TAW-CT127: Under sterile conditions, inoculate Rhodococcus ruber (Rhodococcus ruber) TAW-CT127 into a 1L sterilized Erlenmeyer flask containing 500mL seawater broth culture (2216) , placed in a constant temperature shaker, 28°C, 160rpm, and cultivated for 48h.

[0048] T...

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Abstract

The invention provides a Rhodococcus ruber and application thereof in preparing a decabromodiphenyl oxide degrading agent, relating to application of microbes. The collection number of the Rhodococcus ruber TAW-CT127 is CCTCC NO.M 2015212. The Rhodococcus ruber TAW-CT127 can be used in preparing the decabromodiphenyl oxide degrading agent. The decabromodiphenyl oxide is the unique carbon source in the decabromodiphenyl oxide degradation culture medium. After the Rhodococcus ruber TAW-CT127 is cultured in the degradation culture medium using decabromodiphenyl oxide as the unique carbon source at 28 DEG C under the pH value of 7.4 at the shaking table rotation frequency of 160 rpm, the degradation rate of the Rhodococcus ruber for decabromodiphenyl oxide is up to 91%.

Description

technical field [0001] The invention relates to the application of microorganisms, in particular to a rhodococcus rubrum and its application in the preparation of a decabromodiphenyl ether degradation agent. Background technique [0002] Polybrominated diphenyl ethers (PBDEs) are a kind of brominated flame retardants. As an additive flame retardant, they lack chemical bonds, are easy to dissipate into the air, and can migrate long distances with the atmosphere. PBDEs are difficult to degrade in the external environment, have a long residence time, have strong lipophilicity and hydrophobicity, can be enlarged step by step along the food chain, and can accumulate in protein and adipose tissue in organisms. PBDEs in the atmosphere, water, and soil can pose a threat to the health of humans and advanced organisms through the food chain, and can also migrate over a wide area through the "grasshopper jump effect", leading to global pollution. Studies have confirmed that PBDEs can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01
CPCA62D3/02A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 赵苒蔡庆涛范春王万鹏王碧郭东北王娟闫双双刘敏刘安然胡东
Owner XIAMEN UNIV
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