Check patentability & draft patents in minutes with Patsnap Eureka AI!

Molecular marker of rice spike neck length gene qPNL-12

A molecular marker and panicle neck technology, which is used in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effect of improving breeding and selection efficiency, fast speed and low dosage

Active Publication Date: 2015-08-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above statement, there is no report about the location of rice panicle length-related genes on the 12th chromosome

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker of rice spike neck length gene qPNL-12
  • Molecular marker of rice spike neck length gene qPNL-12
  • Molecular marker of rice spike neck length gene qPNL-12

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Development of a molecular marker closely linked to the new rice panicle neck length gene qPNL-12

[0024] 1) The phenotype of the replacement line C115 and its isolation population construction

[0025] Using a set of high-generation backcross replacement lines with 119 lines constructed with the indica rice variety 9311 as the recipient parent and the japonica rice variety Nipponbare as the donor parent, a very short panicle neck was detected through the investigation of panicle neck length. The replacement line C115 of the phenotype has a very significant difference in the neck length from 9311 ( figure 1a, b), and the plant heights of C115 and 9311 were also significantly different ( figure 1 c), other agronomic traits such as ear length, ear number, grain number per ear and seed setting rate were not significantly different ( figure 1 d-g), the relative length of the inverted I stem node and plant height of C115 has extremely significant difference, and...

Embodiment 2

[0033] Example 2 Polymorphism detection and linkage analysis of closely linked molecular markers with the new rice panicle neck length gene qPNL-12

[0034] 1) Genomic DNA extraction

[0035] At the peak tillering stage of rice, leaf sampling of the rice material to be extracted DNA was carried out. The genomic DNAs of parental 9311, C115 and 30 F2 individual plants were extracted for polymorphism screening of 22 InDel markers; 306 individual plants with short panicle neck phenotype in the F2 population were extracted for linkage analysis of polymorphic markers and fine mapping of qPNL-12 gene; 100 F3 individual plants were extracted for subsequent molecular marker effect verification.

[0036] The genomic DNA of rice leaves was extracted by SDS method, and the operation steps were referred to Della porta SL, et al., Plant Mol Biol Rep, 1983, 1(1):19221.

[0037] The specific steps are: take the leaves of rice seedling stage, grind them with liquid nitrogen in a mortar pre-c...

Embodiment 3

[0045] Example 3 Cloning and sequencing of molecular markers Ind1529 and Ind1561

[0046] The parental 9311 and C115 genomic DNAs were used as templates, and the forward and reverse primers of molecular markers Ind1529 and Ind1561 were used as the primer pairs of Ind1529-F / Ind1529-R and Ind1561-F / Ind1561-R, respectively, and PCR amplification was carried out with high-fidelity enzyme . Take 1 μL of PCR products and connect them with pMD19-T vector for 10 minutes at room temperature, and heat-shock to transform E. coli competent cells DH5α. The transformed bacteria were cultured in 100 ml of LB liquid medium with vulgar shaking for 1 hour, and then spread on 100 μg / ml ampicillin. On the LB solid plate, invert at 37 °C for 14 h. After colony PCR detection, positive clones were selected and sent to Shanghai Yingjun Biological Company for DNA sequence determination.

[0047] The sequencing results showed that the amplification band length of primers Ind1529-F / Ind1529-R in C115 w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker of rice spike neck length gene qPNL-12 which is divided into an upstream molecular marker Ind1529 and a downstream molecular marker Ind1561 respectively aiming to specific insertion / deletion locus polymorphism represented as the SEQ ID No.1 and the SEQ ID No.2. The invention also discloses specific primers comprising SEQ ID No.3, SEQ ID No.4, SEQ ID No.5 and SEQ ID No.6, which are used for amplification on the molecular marker. The molecular marker or the specific primers can be applied in marker assisted selective breeding of the character of spike neck length of a progeny and derivative materials thereof containing the qPNL-12 gene, can save cost and can increase efficiency of breeding and selection.

Description

technical field [0001] The invention belongs to the field of rice genetic improvement and agricultural biotechnology application, and relates to a molecular marker of a rice panicle neck length gene qPNL-12, in particular to a nucleotide sequence closely linked to the qPNL-12 gene molecular marker and a molecule used for amplification Labeled forward and reverse specific primers. Background technique [0002] Ear neck length, also known as ear extraction, refers to the distance from the sheath of flag leaf to the base of the ear. It is an important agronomic trait connecting the stem and ear. plays a very crucial role. Most of the sterile rice lines currently used in production have different degrees of ear-packing characteristics (caused by too short ear extraction), which greatly affects the improvement of hybrid rice seed production. In the 1980s, the discovery of the long panicle neck gene eui in rice was considered to be the fourth factor after the seed production of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
Inventor 赵春芳梁彦丽王才林周丽慧陈涛张亚东赵庆勇
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com