Method for processing hexavalent chromium pollution employing synergistic effects of mixed bacteria
A technology of synergy and compound strains, applied in the field of environmental bioengineering, to achieve the effects of improving governance effects, improving treatment efficiency, and increasing tolerance range
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Embodiment 1
[0033] Acclimatization screening steps:
[0034] (1) Take 10 g of chromium-contaminated soil sample and add it to 100 ml of beef extract-peptone liquid medium, and incubate at 30°C for 24 h with a shaker speed of 120 rpm;
[0035] (2) Take 1 ml of the culture solution in step (1) and transfer it into 100 ml of inorganic salt liquid medium. The medium contains 20 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. Shaker speed is 120 rpm;
[0036] (3) Take 1 ml of the culture solution in step (2) and transfer it to 100 ml of inorganic salt liquid medium. The medium contains 30 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. The rotation speed of the shaker was 120 rpm, and so on, and the rest of the conditions were kept constant. The content of hexavalent chromium was gradually increased in increments of 10 mg / L until the concentration of hexavalent chromium reached 50 mg / L. Aft...
Embodiment 2
[0045] Acclimatization screening steps:
[0046] (1) Take 10 g of chromium-contaminated soil sample and add it to 100 ml of beef extract-peptone liquid medium, and incubate at 30°C for 24 h with a shaker speed of 120 rpm;
[0047] (2) Take 1 ml of the culture solution in step (1) and transfer it into 100 ml of inorganic salt liquid medium. The medium contains 20 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. Shaker speed is 120 rpm;
[0048] (3) Take 1 ml of the culture solution in step (2) and transfer it to 100 ml of inorganic salt liquid medium. The medium contains 30 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. The rotation speed of the shaker was 120 rpm, and so on, and the rest of the conditions were kept constant. The content of hexavalent chromium was gradually increased in increments of 10 mg / L until the concentration of hexavalent chromium reached 50 mg / L. Aft...
Embodiment 3
[0057] Acclimatization screening steps:
[0058] (1) Take 10 g of chromium-contaminated soil sample and add it to 100 ml of beef extract-peptone liquid medium, and incubate at 30°C for 24 h with a shaker speed of 120 rpm;
[0059] (2) Take 1 ml of the culture solution in step (1) and transfer it into 100 ml of inorganic salt liquid medium. The medium contains 20 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. Shaker speed is 120 rpm;
[0060] (3) Take 1 ml of the culture solution in step (2) and transfer it to 100 ml of inorganic salt liquid medium. The medium contains 30 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. The rotation speed of the shaker was 120 rpm, and so on, and the rest of the conditions were kept constant. The content of hexavalent chromium was gradually increased in increments of 10 mg / L until the concentration of hexavalent chromium reached 50 mg / L. Aft...
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