Camera array-based light field microscopic imaging system and method

Abstract

The present invention discloses a camera array-based light field microscopic imaging system and method. The system includes a microscope which enlarges a microscopic sample to an image plane through a cameral lead-out opening, an enlargement module which can generate an aperture plane, an imaging lens array which obtains sample images of the microscopic sample under different angles of view, a sensor array wherein each sensor correspondingly records a sample image obtained by an imaging lens corresponding to the sensor, and a controller which acquires and calibrates the sample images synchronously so as to obtain the light field and / or light field video of the microscopic sample. According to the camera array-based light field microscopic imaging system and method provided by the embodiment of the invention, the sample images of the microscopic sample under different angles of view are obtained and calibrated, so that the light field and / or light field video of the microscopic scene can be obtained, and therefore, high-performance microscopic imaging and application can be realized, and spatial resolution can be improved, and use experience of users can be also improved.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to a camera array-based light field microscopic imaging system and method. Background technique [0002] LFM (Light field microscopy) is a three-dimensional computational imaging method that does not require scanning. This method can simultaneously record the distribution of two-dimensional space and two-dimensional angle of light passing through a microscopic sample. According to the space-angle data, the synthetic refocusing image can be calculated, so as to adjust the depth of field flexibly, and realize the three-dimensional volume reconstruction of the sample, so it has an important application in optical bioimaging. [0003] At present, the light field microscopy imaging method can be realized by adding a microlens array on the intermediate image plane of the optical microscope, so that the sensor pixels can collect light in the light field during a single exposure...

AI Technical Summary

Problems solved by technology

However, MALM (Microlens array based light field microscopy, light field microscopy based on microlens array) needs to make a compromise between the spatial resolution and angular resolution of the sensor, resulting in the spatial resolution of the obtained image compared to the original Sensor resolution is reduced by several orders of magnitude

[0004] In the related art, in order to solve the problem of the reduction of the microscopic spatial resolution of the light field, for example, the spatial resolution of the three-dimensional volume reconstruction can be improved through the method of 3D deconvolution, or the method of additionally adding a phase mask code on the wavefront , so as to eliminate axially uneven spatial resolution, but the computational complexity is high and the cost is high; for example, digital refocusing of the entire sensor resolution can be obtained by means of aperture scanning or LED (Light Emitting Diode, light emitting diode) scanning As a result, time resolution is easily sacrificed; for example, high-resolution light fields can be calculated and reconstructed by introducing sample prior information, such as the Gaussian angle distribution assumption of light field moment imaging, and light field super-resolution with Lambertian reflection coefficient prior rate, by learning a complete dictionary to take advantage of its inherent redundancy method, however these empirical assumptions usually do not hold in microscopic imaging

Therefore, the microscopic imaging method in the related art still fails to solve the problem of low spatial resolution of the light field microscopically, and needs to be improved.

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