Fermented traditional Chinese medicine microbial preparation for grass broodstock and preparation method thereof

Abstract

The invention discloses a fermented traditional Chinese medicine microbial preparation for a parent grass carp and a preparation method of the microbial preparation and relates to a traditional Chinese medicine microbial preparation. The preparation method of the microbial preparation comprises the steps of activating and carrying out enlarged culture on aspergillus oryzae, bacillus subtilis, lactobacillus plantarum and candida utilis, grinding astragalus mongholicus, codonopsis pilosula, bighead atractylodes rhizome, rehmannia glutinosa, Chinese angelica, radix paeoniae alba, pimpinella thellungiana, eucommia, herba epimedii, cuscuta chinensis, a glossy privet fruit, fructus lycii, tangerine peel, rice sprouts and the like to form traditional Chinese medicine powder, preparing soya bean meal, maize cob particles, bran and the like into a solid fermentation medium, putting the solid fermentation medium into a fermentation tank, inoculating aspergillus oryzae seed liquid, candida utilis seed liquid, bacillus subtilis seed liquid and lactobacillus plantarum seed liquid, carrying out aerobic fermentation, and after 36-48h of the fermentation, carrying out anaerobic fermentation to form the fermented traditional Chinese medicine microbial preparation for the parent grass carp. The microbial preparation can be used for antenatal intensive culture and postnatal rapid recovery of the parent grass carp with multiple spawning per year, improves disease resistance of the parent grass carp and increases the egg laying amount.

Description

technical field [0001] The invention relates to a traditional Chinese medicinal microbial preparation, in particular to a fermented traditional Chinese medicinal microbial preparation for grass broodstock and a preparation method thereof. Background technique [0002] Grass carp is the main freshwater aquaculture economic fish in my country and one of the "four major fishes" in China. It has delicious meat, fast growth and low bait requirements, and is deeply loved by consumers. The sexual maturity age of grass carp is 4-7 years old, and the sexual maturity period is long. High-quality grass broodstock is a valuable resource for seed production units. During the spawning process, the physiological damage caused by mechanical damage and excessive physical exertion of the broodstock Sexual physical weakness is the main reason for the decreased disease resistance and high mortality of grass broodstock after delivery. Most fry breeding farms in my country adopt grass carp's onc...

AI Technical Summary

Problems solved by technology

However, the grass broodstock spawns several times a year, and when the second or third spawning occurs, it is in the high temperature season. After the grass broodstock spawns for the first time, the nutrients are exhausted, not only need to recover the same as the normal spawning broodstock , and need to go through the process of nutrient accumulation and gonad re-maturation, and both need to be completed in a short period of time. If the nutritional supplement cannot meet the needs or the grass broodstock is sick and induced labor, after the broodstock is induced again, the broodstock will be weak, injured, etc. The higher mortality rate caused by the above factors, and the indirect problems such as the reduction of spawning rate, fertilization rate and hatching rate, etc., will become more obvious with the increase in the number of times of induced labor. raised higher demands

[0003] At present, postpartum injection of antibiotics is mainly used, and chemical fungicides such as bleaching powder or bait are used to prevent and treat the death of postpartum grass broodstock after entering the pond. It has a certain effect, but drug resistance is becoming more and more prominent with the increase of the age of broodstock. Moreover, problems such as poor quality of fertilized eggs and deformed fish fry indirectly caused by this cannot be ignored.

The postpartum glucose injection of grass broodstock, the method of increasing the feeding amount of tender grass and supplementing bean cake, malt and other concentrates after entering the pond has a certain effect on the postpartum physical recovery of grass broodstock, but the recovery period is as long as 20 days or more. If the requirements for secondary induced production are met, the recovery and intensive cultivation period is often longer than 50-70 days, which affects and shortens the growth period of the secondary hatching fry, and the size of the fry overwintering is too small, which directly affects the survival rate and economic benefits. The growth period of northern grass carp is relatively short, so the multiple spawning technique of grass carp has no economic value because the second spawning date is too late

[0004] Microorganisms such as Bacillus, lactic acid bacteria, and yeast have significant effects on improving water quality, increasing feed remuneration, promoting fish growth, and enhancing fish disease resistance, but there are also probiotics with unstable numbers, low activity, and intestinal colonization. Weak and other shortcomings

Traditional Chinese herbal medicine in my country is widely used in the prevention and treatment of fish diseases, the promotion of animal gonad development, and postpartum recovery. It has the advantages of safety, small toxic and side effects, green and natural, and no drug residues. Poor, troublesome to use, high extraction cost and other disadvantages

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