Unlock instant, AI-driven research and patent intelligence for your innovation.

Tuna dark meat protein anti-cervical cancer polypeptide

A cervical cancer and tuna technology, applied in the field of tuna dark meat protein anti-cervical cancer peptides

Active Publication Date: 2020-10-20
德州蓝力生物技术有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The applicant's research found that the preparation of anti-cervical cancer polypeptides using enzymatic hydrolysis technology using the dark meat of tuna as raw materials is still in a blank stage, and there is no report on the preparation of highly active anti-cervical cancer polypeptides using enzymatic hydrolysis products as materials.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tuna dark meat protein anti-cervical cancer polypeptide
  • Tuna dark meat protein anti-cervical cancer polypeptide
  • Tuna dark meat protein anti-cervical cancer polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] 1) Pretreatment of dark-colored tuna meat: homogenate the dark-colored tuna meat, heat it to 95°C and keep it warm for 10 minutes, then cool it down to room temperature, add isopropanol according to the ratio of solid to liquid 1g:5mL, degrease at room temperature for 24 hours, and then place it at 4°C, 10000rpm centrifuge 20min to remove isopropanol, collect the solid matter of defatted tuna dark meat, which is the defatted tuna dark meat protein; the tuna is preferably skipjack.

[0014] 2) Enzymolysis of defatted tuna dark meat protein: take defatted tuna dark meat protein as raw material, add Gly-NaOH buffer solution (0.05mol / L, pH 9.5) according to the solid-to-liquid ratio of 1g:25mL, to obtain a mixed solution; mix the mixed solution Preheat the temperature to 50°C for 10 minutes, add protease according to 2% of the mass of the dark meat of defatted tuna, and enzymatically hydrolyze at 50°C. After 4 hours of enzymatic hydrolysis, raise the temperature of the solut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a tuna dull color meat protein anti-cervical carcinoma polypeptide with the amino acid sequence of Gln-Tyr-Asp-Glu-Tyr-Trp, and the molecular weight detected by ESI-MS is 902.88 Da. The tuna dull color meat protein anti-cervical carcinoma polypeptide has significant inhibitory effect on cell proliferation of human cervical carcinoma cell line, the IC50 is 0.16 mg / mL, and the tuna dull color meat protein anti-cervical carcinoma polypeptide can be used for the preparation of drugs for the prevention and control of cervical carcinoma.

Description

technical field [0001] The invention relates to a fish functional polypeptide, in particular to a tuna dark meat protein anti-cervical cancer polypeptide. Background technique [0002] Tuna is an oceanic fish, and it is a key target fish species for the development of pelagic fisheries in the world. It is also recommended by the International Society of Nutrition as one of the world's three most nutritious fish. According to the statistics of the Food and Agriculture Organization of the United Nations, the total output of oceanic fisheries in the world is 8.5 million tons, of which the output of tuna exceeds 6 million tons, accounting for more than 70% of the total output of high seas fisheries. A large amount of dark flesh is produced during tuna processing, accounting for about 11% of the raw material. Existing studies have shown that the dark meat of tuna is rich in essential amino acids, comprehensive, easy to digest and absorb, and is a high-quality raw material for pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61P35/00
Inventor 迟长凤王斌赵玉勤孙坤来
Owner 德州蓝力生物技术有限公司