Primer and probe for on-site rapid detection of mycobacterium tuberculosis complex and kit thereof

A technology of Mycobacterium tuberculosis, complex group, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of high false positive rate, poor specificity, low sensitivity, etc. Convenience, simple detection, high sensitivity effect
CN104862406AActive Publication Date: 2015-08-26DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
Publication Date
2015-08-26

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Abstract

The invention discloses a primer and a probe for on-site rapid detection of mycobacterium tuberculosis complex and a kit thereof. The forward primer sequence is shown as SEQ ID No.1, the reverse primer sequence is shown as SEQ ID No.2, and the probe sequence is shown as SEQ ID No.3. The invention provides the PA-LFD primer and the probe for on-site distinctive, sensitive, simple and rapid detection of the mycobacterium tuberculosis complex (mycobacterium tuberculosis, mycobacterium bovis, African mycobacteria and Canna mycobacteria) and the kit containing the primer and the probe.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying Mycobacterium tuberculosis complex using Recombinase Polymerase Amplification and Lateral Flow Dipstick (RPA-LFD) detection technology and related Primers and probes and detection kits. Background technique

[0002] The pathogenic bacteria in the Mycobacterium tuberculosis complex (Mycobacterium tuberculosis complex, MTC) that can cause tuberculosis in humans and animals include Mycobacterium tuberculosis (M.tuberculosis), Mycobacterium bovis (M.bovis), Mycobacterium africa (M.africanum), Mycobacterium canneri (M.canettii), etc. Tuberculosis is a zoonotic infectious disease that seriously endangers the health of humans and animals. The number of deaths due to tuberculosis is about 2 million to 3 million worldwide every year. According to the statistics of the World Health Organization, my country is one of the 22 countries with severe tuberculos...

Claims

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