A method for extracting high-content α-mangostin from mangosteen bark

Abstract

The invention discloses a method for extracting high-content alpha-mangostin from mangosteen skin. The method comprises the following steps: (1) extracting: crushing mangosteen skin raw materials, placing the crushed mangosteen skin raw materials into an extracting device, adding an acid alcohol solvent in amount which is 4 to 5 times the weight of the mangosteen skin raw materials, placing the solution into water bath at the temperature of 85-95 DEG C, extracting for 2 to 3 times and for 1.5 to 2 hours at each time, combining the extracting liquids and filtering to obtain the filtered extracting liquid; and (2) performing chromatography: allowing the filtered liquid to pass through a neutral alumina chromatographic column, performing gradient elution, collecting the eluent, concentrating the eluent under reduced pressure until no alcohol taste exists, standing the eluent at the temperature of 4 DEG C for more than or equal to 4 hours until crystallization, centrifuging, and taking the precipitate to obtain the finished product. The method has the advantages of few steps, low time consumption, little reagent dosage and low cost, and is simple to operate and easy to control. By the method, the alpha-mangostin with the highest content of more than or equal to 99.0% can be obtained. The method is suitable for industrialized production.

Description

technical field

[0001] The invention relates to a method for extracting high-content α-mangostin from mangosteen bark, which belongs to the technical field of natural plant extraction. Background technique

[0002] Mangosteen (Garcinia mangostana Linn.), also known as mangosteen, mangostana, mangosteen, mangosteen, phoenix fruit, is the fruit of mangosteen, an evergreen tree of the genus Garcinia. It is native to the Malay Peninsula and the Malay Archipelago, and is more cultivated in Southeast Asia such as Malaysia, Thailand, the Philippines, and Myanmar.

[0003] Studies have found that mangosteen peel contains a large amount of xanthones (xanthones), which have anti-inflammatory, anti-tumor, anti-oxidation and other activities. Among them, the most important xanthone compounds are α-, β-, γ-mangostin, garcinone and so on. The most abundant xanthone compound in mangosteen shell is α-mangostin.

[0004] α-Mangostin, the chemical name is 1,3,6-trihydroxy-7-methoxy-2,8-bis...

Examples

Embodiment 1

[0026] (1) Extraction: 0.8mL of analytically pure hydrochloric acid is dissolved in 70% ethanol by volume percentage to obtain a volume percentage of 1‰ acid alcohol solvent, and the raw material of 100g mangosteen bark is taken, and the total content of its α-mangostin is analyzed by HPLC It was detected as 3.2%. After crushing, put it in the extraction tank, then add 400mL of the above-mentioned acid-alcohol solvent, place it in a 90°C water bath, and extract for 2 hours to obtain the first extract, and then add 400mL of the above-mentioned Acid-alcohol solvent, placed in a 90°C water bath, extracted for 1.5 hours to obtain the second extract, combined the two extracts and passed through a 200-mesh filter cloth to obtain 794 mL of the filtered extract;

[0027] (2) Chromatography: get the neutral alumina chromatography column, the weight of alumina in the chromatography column is 200g, then the extract after step (1) gained after filtering, cross above-mentioned neutral alumi...

Embodiment 2

[0043] (1) Extraction: 0.5mL analytically pure formic acid is dissolved in 80% ethanol by volume percentage to obtain a volume percentage of 0.5‰ acid alcohol solvent, get 100g mangosteen bark raw material, and the total content of its α-mangostin is determined by HPLC It was detected as 3.3%. After crushing, put it in an extraction bottle, add 500mL of the above-mentioned acid-alcohol solvent, place it in a water bath at 95°C, and extract for 1.5h to obtain the first extract, and then add 500mL of Put the above acid-alcohol solvent in a water bath at 85°C and extract for 1.5 hours to obtain the second extract, combine the two extracts and pass through a 100-mesh filter cloth to obtain 994 mL of the filtered extract;

[0044](2) Chromatography: get the neutral alumina chromatography column, the weight of alumina in the chromatography column is 150g, then the extract after step (1) gained after filtering, cross above-mentioned neutral alumina chromatography column Finally, carr...

Embodiment 3

[0048] (1) Extraction: 0.8mL of analytically pure sulfuric acid is dissolved in 75% ethanol by volume percentage to obtain a volume percentage of 0.8‰ acid alcohol solvent, and the raw material of 100g mangosteen bark is taken, and the total content of its α-mangostin is determined by HPLC It was detected as 3.15%. After crushing, put it in the extraction tank, then add 450mL of the above-mentioned acid-alcohol solvent, place it in a water bath at 85°C, and extract for 2 hours to obtain the first extract, then add 450mL of the above-mentioned Acid-alcohol solvent, placed in a 95°C water bath, extracted for 1.5h, to obtain the second extract, then 450mL of the above-mentioned acid-alcohol solvent was added to the extraction tank, placed in a 90°C water bath, extracted for 1.5h, to obtain the third The extract of the third time was combined for three times and passed through a 150-mesh filter cloth to obtain 1334 mL of the filtered extract;

[0049] (2) Chromatography: get the n...

technical field

[0001] The invention relates to a method for extracting high-content α-mangostin from mangosteen bark, which belongs to the technical field of natural plant extraction. Background technique

[0002] Mangosteen (Garcinia mangostana Linn.), also known as mangosteen, mangostana, mangosteen, mangosteen, phoenix fruit, is the fruit of mangosteen, an evergreen tree of the genus Garcinia. It is native to the Malay Peninsula and the Malay Archipelago, and is more cultivated in Southeast Asia such as Malaysia, Thailand, the Philippines, and Myanmar.

[0003] Studies have found that mangosteen peel contains a large amount of xanthones (xanthones), which have anti-inflammatory, anti-tumor, anti-oxidation and other activities. Among them, the most important xanthone compounds are α-, β-, γ-mangostin, garcinone and so on. The most abundant xanthone compound in mangosteen shell is α-mangostin.

[0004] α-Mangostin, the chemical name is 1,3,6-trihydroxy-7-methoxy-2,8-bis...

Examples

Embodiment 1

[0026] (1) Extraction: 0.8mL of analytically pure hydrochloric acid is dissolved in 70% ethanol by volume percentage to obtain a volume percentage of 1‰ acid alcohol solvent, and the raw material of 100g mangosteen bark is taken, and the total content of its α-mangostin is analyzed by HPLC It was detected as 3.2%. After crushing, put it in the extraction tank, then add 400mL of the above-mentioned acid-alcohol solvent, place it in a 90°C water bath, and extract for 2 hours to obtain the first extract, and then add 400mL of the above-mentioned Acid-alcohol solvent, placed in a 90°C water bath, extracted for 1.5 hours to obtain the second extract, combined the two extracts and passed through a 200-mesh filter cloth to obtain 794 mL of the filtered extract;

[0027] (2) Chromatography: get the neutral alumina chromatography column, the weight of alumina in the chromatography column is 200g, then the extract after step (1) gained after filtering, cross above-mentioned neutral alumi...

Embodiment 2

[0043] (1) Extraction: 0.5mL analytically pure formic acid is dissolved in 80% ethanol by volume percentage to obtain a volume percentage of 0.5‰ acid alcohol solvent, get 100g mangosteen bark raw material, and the total content of its α-mangostin is determined by HPLC It was detected as 3.3%. After crushing, put it in an extraction bottle, add 500mL of the above-mentioned acid-alcohol solvent, place it in a water bath at 95°C, and extract for 1.5h to obtain the first extract, and then add 500mL of Put the above acid-alcohol solvent in a water bath at 85°C and extract for 1.5 hours to obtain the second extract, combine the two extracts and pass through a 100-mesh filter cloth to obtain 994 mL of the filtered extract;

[0044](2) Chromatography: get the neutral alumina chromatography column, the weight of alumina in the chromatography column is 150g, then the extract after step (1) gained after filtering, cross above-mentioned neutral alumina chromatography column Finally, carr...

Embodiment 3

[0048] (1) Extraction: 0.8mL of analytically pure sulfuric acid is dissolved in 75% ethanol by volume percentage to obtain a volume percentage of 0.8‰ acid alcohol solvent, and the raw material of 100g mangosteen bark is taken, and the total content of its α-mangostin is determined by HPLC It was detected as 3.15%. After crushing, put it in the extraction tank, then add 450mL of the above-mentioned acid-alcohol solvent, place it in a water bath at 85°C, and extract for 2 hours to obtain the first extract, then add 450mL of the above-mentioned Acid-alcohol solvent, placed in a 95°C water bath, extracted for 1.5h, to obtain the second extract, then 450mL of the above-mentioned acid-alcohol solvent was added to the extraction tank, placed in a 90°C water bath, extracted for 1.5h, to obtain the third The extract of the third time was combined for three times and passed through a 150-mesh filter cloth to obtain 1334 mL of the filtered extract;

[0049] (2) Chromatography: get the n...

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