Application of bufexamac in preparation of bactericide used for controlling plant disease caused by phytopathogen
A technology of plant pathogenic bacteria and butylated hydroxy acid, which is applied in the field of fungicides for plant diseases, and can solve problems such as reports on the inhibitory effect of butylated hydroxy acid
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[0035] 1. Experimental materials
[0036] Butyric acid is a finished product purchased from Fluka. Weigh 4.47g of butyl benzene hydroxy acid in 20ml of dimethyl sulfoxide to make a 1M mother liquor, filter and sterilize and store at 4°C until use.
[0037] The plant pathogens used in this experiment are strains stored at 4°C in the laboratory, and the medium used is potato medium (PDA for short).
[0038] PDA medium formula: 200g potato (peeled), 20g glucose, 15g agar, 1000ml tap water, natural PH
[0039] Preparation method: Wash and peel the potatoes, weigh 200g and cut into small pieces, add water to boil (boil for 20-30 minutes, can be pierced by a glass rod), filter in a beaker with eight layers of gauze, add according to the needs of the experiment 15-20g agar, add 20g glucose, stir evenly, fully dissolve and cool to make up water to 1000ml, sterilize at 121°C for 20 minutes after aliquoting, and cool for use.
[0040] 2. Experimental method
[0041] Use the growth rate method.
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