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Preparation method of radial collagen scaffold in combination with stromal cell derived factor and application of radial collagen scaffold in combination with stromal cell derived factor to osteochondral defect repair

A technology of deriving factors and stromal cells, applied in medical science, prosthesis, etc., can solve problems such as unfavorable cell migration and affecting cell growth

Inactive Publication Date: 2015-09-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the three-dimensional structure and porosity of the material can affect the growth of cells in it, most of the current material structures are disordered and not conducive to cell migration, so it is of great significance to design a radial material

Method used

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  • Preparation method of radial collagen scaffold in combination with stromal cell derived factor and application of radial collagen scaffold in combination with stromal cell derived factor to osteochondral defect repair
  • Preparation method of radial collagen scaffold in combination with stromal cell derived factor and application of radial collagen scaffold in combination with stromal cell derived factor to osteochondral defect repair
  • Preparation method of radial collagen scaffold in combination with stromal cell derived factor and application of radial collagen scaffold in combination with stromal cell derived factor to osteochondral defect repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Scaffold preparation and detection:

[0053](1) Preparation of radial collagen stents: radial collagen stents were produced using a radial collagen stent device. The radial collagen stent device was a mold with an inner diameter of 5.0 mm and a height of 20.0 mm. The mold was cylindrical and frozen at -20°C as a freezing source. There is a plastic cylinder with a diameter of 1.5mm in the center of the mold, and the top and bottom of the mold are made of plastic. Add the aqueous collagen solution into the mold and continue to freeze at -20°C. The aqueous collagen solution gradually crystallizes from the outside of the mold to the inside. When the collagen aqueous solution in the entire mold is completely crystallized, place the radial collagen scaffold device at -80°C until the temperature of the radial collagen scaffold drops to -80°C, and vacuum-dry the radial collagen scaffold device for 48 hours to obtain a collagen scaffold. A schematic diagram of the pr...

Embodiment 2

[0058] Preparation method of stromal cell-derived factor combined with radial collagen scaffold and its application in osteochondral defect repair

[0059] 1. Animal model establishment and scaffold implantation

[0060] ① Anesthesia: Calculated at 40 mg / kg, intraperitoneally anesthetized with 1% sodium pentobarbital.

[0061] ②Preparation of the rabbit knee joint skin in a sterile environment: Disinfect with 2% iodophor, cut an incision about 1 cm along the medial edge of the patellar ligament, cut the knee joint layer by layer, and rotate it on the femoral pulley with a graduated hand Drill the osteochondral defect with a diameter of 4mm and a depth of 5mm to complete the modeling.

[0062] 2. Grouping of animal experiments:

[0063] All 18 New Zealand white rabbits were modeled, and were randomly divided into 3 groups after making defects: traditional collagen scaffold group, radial collagen scaffold group and radial collagen scaffold group containing SDF-1, 6 rabbits in ...

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Abstract

The invention discloses a preparation method of a radial collagen scaffold in combination with stromal cell derived factor (SDF) and application of the radial collagen scaffold in combination with stromal cell derived factor to osteochondral defect repair. The preparation method comprises the steps of preparing a radial collagen scaffold by use of a radial collagen scaffold device, preparing a collagen water solution and preparing a radial collagen scaffold in combination with SDF-1. According to the application of SDF-1-containing radial collagen scaffold, which can promote autologous cell migration, to osteochondral defect repair, migration of stem cells is promoted in two aspects. Most conventional materials are disordered in structure, while osteochondral defect repair needs a material which can promote migration of mesenchymal stem cells. The preparation method of the SDF-1-containing radial collagen scaffold is made clear, in-vivo functional check is realized, and a basis is laid for clinical application of the SDF-1-containing radial collagen scaffold.

Description

technical field [0001] The invention designs the repair of osteochondral defect, and particularly relates to the application of a matrix cell-derived factor combined with a radial collagen scaffold in the repair of osteochondral defect. Background technique [0002] Osteochondral defect is an important pathological change that occurs in the late stage of osteoarthritis (OA). Osteoarthritis is the most common orthopedic disease and causes huge medical costs. Therefore, the World Health Organization launched the second "Osteoarthritis 10 Years" plan to fight OA. Since articular cartilage lacks self-repair ability, the repair of articular cartilage defects is the biggest challenge in the clinical treatment of osteoarthritis. So far, autologous chondrocyte transplantation is the first choice for the clinical treatment of osteochondral defects. Although its curative effect is definite, its surgical indications are limited. 80% of patients finally choose joint replacement surgery,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/54A61L27/50
Inventor 陈鹏飞欧阳宏伟陶佳栋
Owner ZHEJIANG UNIV
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