Open tubular capillary column with nano-crystalline cellulose derivative modified surface and application of open tubular capillary column with nano-crystalline cellulose derivative modified surface

An open-tube capillary column and nanocellulose technology, which is applied in other chemical processes, solid adsorbent liquid separation, instruments, etc., can solve the problems of chiral separation of nanocellulose, achieve satisfactory separation effect, and simple preparation method , low cost effect

Active Publication Date: 2015-09-30
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, there are no related reports on the application of nanocellulose as a capillary electrophoresis tube wall modifier for chiral resolution

Method used

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  • Open tubular capillary column with nano-crystalline cellulose derivative modified surface and application of open tubular capillary column with nano-crystalline cellulose derivative modified surface
  • Open tubular capillary column with nano-crystalline cellulose derivative modified surface and application of open tubular capillary column with nano-crystalline cellulose derivative modified surface
  • Open tubular capillary column with nano-crystalline cellulose derivative modified surface and application of open tubular capillary column with nano-crystalline cellulose derivative modified surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 10 g of microcrystalline cellulose was hydrolyzed with 64% concentrated sulfuric acid, and after several times of centrifugation, the unhydrolyzed cellulose was removed to obtain nanocellulose, which was about 3 g after freeze-drying; 3 g of nanocellulose was protected by 6-position Methods Modification of 3,5-dimethylphenylisocyanate on nanocellulose. Activate the capillary with 0.1 mol / L NaOH, distilled water, 0.1 mol / L HCl, and distilled water in sequence. Then fix the treated capillary on the pump head, and at a flow rate of 0.1mL / min, first pass 10 mol / L cetyltrimethylammonium bromide into the capillary, and adsorb on the inner wall of the capillary by electrostatic action, Then use N 2 Purge until no solution flows out; then dissolve the above-prepared nanocellulose derivatives in pyridine and add them to the ethanol solution of ethyl orthosilicate, the concentration of nanocellulose derivatives is 0.1 mg / mL, and stir under ice bath conditions After 4 hours of r...

Embodiment 2

[0024] 10 g of microcrystalline cellulose was hydrolyzed with 64% concentrated sulfuric acid, and after several times of centrifugation, the unhydrolyzed cellulose was removed to obtain nanocellulose, which was about 3 g after freeze-drying; 3 g of nanocellulose was protected by 6-position Methods Modification of 3,5-dimethylphenylisocyanate on nanocellulose. Activate the capillary with 0.1 mol / L NaOH, distilled water, 0.1 mol / L HCl, and distilled water in sequence. Then fix the treated capillary on the pump head, and at a flow rate of 0.2 mL / min, first pass 20 mol / L cetyltrimethylammonium bromide into the capillary, and adsorb on the inner wall of the capillary by electrostatic action, Then use N 2 Purge until no solution flows out; then dissolve the above-prepared nanocellulose derivatives in pyridine and add them to the ethanol solution of ethyl orthosilicate, the concentration of nanocellulose derivatives is 0.2 mg / mL, and stir under ice bath conditions After 4 hours of ...

Embodiment 3

[0026] 10 g of microcrystalline cellulose was hydrolyzed with 64% concentrated sulfuric acid, and after several times of centrifugation, the unhydrolyzed cellulose was removed to obtain nanocellulose, which was about 3 g after freeze-drying; 3 g of nanocellulose was protected by 6-position Methods Modification of 3,5-dimethylphenylisocyanate on nanocellulose. Activate the capillary with 0.1 mol / L NaOH, distilled water, 0.1 mol / L HCl, and distilled water in sequence. Then fix the treated capillary on the pump head, and at a flow rate of 0.3 mL / min, first pass 40 mol / L cetyltrimethylammonium bromide into the capillary, and adsorb on the inner wall of the capillary by electrostatic action, Then use N 2 Purge until no solution flows out; then dissolve the above-prepared nanocellulose derivatives in pyridine and add them to the ethanol solution of ethyl orthosilicate, the concentration of nanocellulose derivatives is 0.4 mg / mL, and stir under ice bath conditions After 4 hours of ...

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Abstract

The invention discloses an open tubular capillary column with a nano-crystalline cellulose derivative modified surface. The open tubular capillary column is prepared by the step: modifying and deriving nano-crystalline celluloses of 3,5-dimethylphenyl isocyanate on the wall of a bare capillary column subjected to activation by adopting an in-situ hybridization method. The open tubular capillary column has the advantages that the preparation is easy and a coating is stable, and can be used for capillary electrophoresis separation analysis on an amino acid chiral enantiomer.

Description

technical field [0001] The invention relates to an open-tube capillary column of surface-modified nano-cellulose derivatives and an application thereof. Background technique [0002] Chirality is a ubiquitous phenomenon in nature, and chiral enantiomers with different structures have huge differences in pharmacological activity and other aspects (such as toxicity, adverse reactions, etc.). Cellulose is an inexhaustible renewable natural polymer in nature. It is the most readily available natural optically active polymer, and it is easy to modify or derivatize. It has a special chiral recognition ability. It is used as a chiral stationary phase Rapid development and wide application. At present, many scientific research institutions at home and abroad have made great progress in the research of cellulose chiral resolving agents, and a batch of cellulose stationary phase products have been developed into commercial columns. [0003] In recent years, with the in-depth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447B01D15/22B01J20/30B01J20/285B01J20/291
Inventor 赵亮董树清孙亚明毕青王利涛张霞张晓莉
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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