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Seed selection method for stichopus japonicas seeds with protosalt resistance

The technology of salt sea cucumber and seed is applied in the field of breeding of low-salt-tolerant sea cucumber seed, which can solve the problems of complex process, unstable low-salt tolerance of seed, etc. The effect of improving industrial output and economic benefits

Active Publication Date: 2015-10-07
MARINE BIOLOGY INST OF SHANDONG PROVINCE
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Problems solved by technology

In addition, the current risk of sea cucumber farming in low-salt sea areas is much higher than that in suitable sea areas. For example, in the summer of 2013, my country experienced abnormal climate with concentrated heavy rainfall and continuous high temperature, which caused damage to sea cucumber farming in low-salt sea areas such as the Yellow River Delta. The rate of damage is over 80%, while the rate of damage in suitable sea areas is about 20%.
In recent years, Chinese scientific and technological personnel have successively carried out the cultivation of low-salt-tolerant Apostichopus japonicus seedlings, and initially obtained seedlings that can grow at a salinity of 19-20°C. Due to the disadvantages of decreasing domestication and breeding, the low-salt tolerance of seedlings is extremely unstable, and it has not yet been popularized and applied in actual production.

Method used

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  • Seed selection method for stichopus japonicas seeds with protosalt resistance

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specific Embodiment

[0035] (1) On March 20, 2009, sea cucumbers that had been cultured for more than 1.5 years and had an individual weight of more than 300g / head were selected from a breeding pond in a certain area of ​​the Yellow River Delta in Shandong as the basic population for breeding, and moved to industrialized breeding ponds separately. For temporary storage, the water in the temporary storage pool is mixed with natural seawater and underground well water to adjust the salinity to 20±1.5 and placed for 48 hours before use. Keep the pH value at 7.8-8.5, the dissolved oxygen above 5mg / L, and the temperature within 16°C. Feed the compound feed at 1% to 3% of the body weight of sea cucumbers every day, and stop 5 days before induced labor.

[0036] (2) On May 12, select individuals who still eat and grow normally and have strong vitality, and use the method of drying in the shade + running water stimulation to artificially induce labor. The egg-laying water temperature is 20°C and the salini...

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Abstract

The invention provides a seed selection method for stichopus japonicas offspring seeds with protosalt resistance. The seed selection method is characterized in that in the late March, and before sea water temperature going below 12 DEG C, local stichopus japonicas bred for over 1 year and a half and weighing over 250g each are selected from a river port low-salt area to be a seed selection base group and separately bred in a pond for a while; by May with flood season salinity around 18.8 to 21.5 and temperature 16 DEG C, stichopus japonicas which can still ingest and grow are chosen to be seed stichopus japonicas, then artificially bred under the environment with salinity around 18.8 to 21.5 to achieve F1 offspring seeds; mature F1 offspring individuals after two years breeding then respectively conduct selfing and crossbreed with the selected base group to achieve F2 offspring; breed and growth of the F2 offspring are conducted under a condition with salinity around 18.5 to 21.5; the above phases are circulated, after three to four accumulated selection, stichopus japonicas seeds with protosalt resistance and stable inheritable characters can be achieved; breeding range can be enlarged; product quality and output can be enhanced; and production risks can be reduced and economic benefits of fishermen can be improved.

Description

technical field [0001] The invention relates to seawater culture technology, in particular to a breeding method for low-salt-tolerant sea cucumber seedlings. Background technique [0002] Apostichopus japonicus is a kind of narrow-saline animal, and the suitable growth salinity range is 26-35. In my country, it is mainly distributed in the yellow sea and Bohai sea with suitable salinity in Liaoning, Shandong and Hebei. In order to further expand the sea cucumber breeding space, expand the breeding scale, and increase production to meet the growing consumer demand, Shandong Province has taken the lead in conducting breeding experiments in the low-salt sea area of ​​the Yellow River Delta where there is no natural distribution of sea cucumbers since 2003. In 2005, it was successful and proposed The development strategy of "East ginseng and West cultivation" has been adopted. At present, the area of ​​pond culture has been developed to about 337,000 mu, accounting for 69.5% of ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 李成林胡炜赵斌韩莎
Owner MARINE BIOLOGY INST OF SHANDONG PROVINCE
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