Antibody antigen detection method based on mobile device

A technology for antigen detection and mobile equipment, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of complex operation, long detection time and high cost, and achieve the effects of high detection accuracy, fast detection speed and easy operation.

Active Publication Date: 2016-08-17
微奥云(深圳)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the detection time is very long, and it often takes a day or even several days from collecting samples to obtaining results
At the same time, the testing personnel need professional training, and the testing results are related to the experience of the testing personnel, which has a certain degree of subjectivity
[0008] In summary, the current problems of immunoassays are: complex operation, many antibody / antigen immunoassays require laboratory environment and special training for testing personnel, the test results are subjective, the test speed is slow, the cost is high, and the test results are inconvenient archive

Method used

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  • Antibody antigen detection method based on mobile device
  • Antibody antigen detection method based on mobile device
  • Antibody antigen detection method based on mobile device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Such as figure 1 with figure 2 As shown, an antibody antigen detection and test system includes at least one reaction unit 1. The reaction unit 1 includes a reaction chamber with an opening at the top. A detection plate is provided at the bottom of the reaction chamber, and at least a pair of electrode sheets 11 are laid on the detection plate. , The terminal 12 of the electrode sheet 11 passes through and is fixed on the box body of the reaction chamber; the detection plate is also fixed with the target antibody or the corresponding antigen or antibody of the antigen.

[0053] Immobilization of the target antibody or the corresponding antigen or antibody of the antigen is the coating process of the antigen or antibody. The target antibody or antigen in the body fluid sample to be tested can be better fixed on the detection plate after binding to the corresponding antigen or antibody coated on the surface of the electrode sheet, which is beneficial to improve the accuracy ...

Embodiment 2

[0068] Such as Figure 3-Figure 5 As shown, a method for detecting mastitis using the antibody antigen detection and test system of Example 1, adopts the following steps:

[0069] 1) Determine the excitation signal parameters of the target antibody or antigen; use 10Hz~10MHz signal to scan the target antibody or antigen in the laboratory, observe the response of the target antibody or antigen, and find the strongest response of the target antibody or antigen The signal parameter is determined as the excitation signal parameter of the target antibody or antigen;

[0070] In specific implementation, such as image 3 As shown, the test solution containing mastitis antibody is scanned with a signal of 10Hz~10MHz, and the capacitance change rate in the test solution is detected. It is found that when the signal frequency is closer to 100kHz, the capacitance change rate in the test solution is greater. , It is determined that the amplitude of the excitation signal of mastitis antibody i...

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Abstract

The present invention discloses an antibody / antigen detection method based on mobile equipment. The method comprises: 1) determining the excitation signal parameter of a target antibody or antigen; 2) acquiring a reaction unit, wherein the reaction unit comprises an electrode pair spread on the bottom portion of the reaction unit; 3) acquiring auxiliary detecting control modules, wherein the modules comprise an excitation signal conditioning module; 4) sampling and pretreating a body fluid sample to be detected; 5) applying an excitation signal and sampling an impedance value; and 6) carrying out data analysis to obtain the detection result, wherein mobile intelliegnt equipment is used to read the impedance value stored in the auxiliary detecting control module, the read impedance value is analyized through the detecting control software arranged in the mobile intelliegnt equipment so as to qualitatively obtain the detection result, and the detection result is uploaded to the cloud terminal. The method of the present invention has advantages of easy operation, rapid detection, high detection precision, and the like.

Description

Technical field [0001] The invention relates to the field of immunoassay detection, and in particular to a mobile device-based antibody antigen detection method. Background technique [0002] The electric double layer means that any two different objects contact will generate an electric potential between the two phases. Each of the two phases has an excess charge, the electric quantity is equal, the sign is opposite, and the phase is attracted to form an electric double layer. There is a potential at the metal-electrolyte two-phase interface of the electrode, which will also produce an electric double layer. When a metal electrode is inserted into a solution, the behavior of the electrode / solution interface is similar to that of a flat capacitor, and it can be described by a physical equation similar to that of a capacitor: [0003] [0004] Where C is the interface capacitance, Is the vacuum dielectric constant, Is the dielectric constant of the electrode / solution interface ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/561
CPCG01N33/561
Inventor 刘晓竹
Owner 微奥云(深圳)生物技术有限公司
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