Unlock instant, AI-driven research and patent intelligence for your innovation.

Nickel-IgG chelate as well as preparation method and application thereof

A chelate and chelation reaction technology, applied in the field of detection, can solve problems such as nickel pollution, and achieve the effect of improving repeatability and accuracy

Inactive Publication Date: 2015-10-21
上海拜豪生物科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For the serious problem of nickel pollution, the object of the present invention is to provide a kind of nickel-IgG chelate and preparation method thereof, and establish the qualitative and quantitative detection method of nickel-IgG chelate, so that quantitatively detect nickel-IgG chelate Application in evaluating nickel pollution degree in an area

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nickel-IgG chelate as well as preparation method and application thereof
  • Nickel-IgG chelate as well as preparation method and application thereof
  • Nickel-IgG chelate as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0059] The present invention also provides a kind of preparation method of nickel-IgG chelate, comprises the following steps:

[0060] A) the chelation reaction of nickel and IgG: adding nickel ions to human IgG or IgG recombined according to biological methods for chelation reaction to obtain a reaction solution;

[0061] B) Extraction of purified nickel-IgG chelates: Immunoaffinity chromatography is used to remove unreacted IgG and excess nickel ions in the reaction solution to obtain nickel-IgG chelates. The specific steps are as follows:

[0062] (1) Dissolving sample: add physiological saline to the reaction solution obtained through step A) to redissolve the nickel-IgG chelate

[0063] (2) Equilibrium chromatography column: Use the dilution buffer to flush the pipeline of the chromatography column, fill the chromatography column with a filler that can specifically bind to IgG, and continue to use the dilution buffer to balance the chromatography column after loading the ...

Embodiment 1

[0157] Example 1 : the preparation method of nickel-IgG chelate, comprises the following steps:

[0158] A) the chelation reaction of nickel and IgG: adding nickel ions to the IgG of human origin for chelation reaction to obtain a reaction solution;

[0159] Reagent preparation:

[0160] 1) Borate buffer (0.01M): Weigh 0.31g of boric acid and dissolve it in 400ml of ultrapure water, adjust the pH to 9.0 with 0.1M NaOH, and dilute to 500mL.

[0161] 2) IgG solution: Weigh 4.0mg IgG and dissolve it in 4.0mL 0.01M pH9.0 borate buffer solution, fully shake and dissolve, and prepare a 1.0mg / mL protein solution;

[0162] 3) 5mmol / L EDTA+200mmol / LNaHCO 3 Solution: weigh EDTA·2H 2 O 1.86g, NaHCO 3 Dissolve 16.8g in 900mL ultrapure water, adjust the pH to 8.0 with 1.0M NaOH and dilute to 1000ml, autoclave, and store at room temperature;

[0163] 4) ITCBE (purchased from Japan Tongren Chemical Research Institute, item number M030)

[0164] 5) Dialysis bag (molecular weight cut o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a nickel-IgG chelate as well as a preparation method and application thereof. The nickel-IgG chelate is formed by chelating nickel ions with IgG through sulfydryl or / and cysteine residues. According to the invention, a qualitative and quantitative detection method of the nickel-IgG chelate is established for favorably quantitatively detecting the application of the nickel-IgG chelate to the evaluation of the nickel pollution degree of one region. By quantitatively detecting the nickel-IgG chelates in serums of people in one region, the condition that people in the region are polluted by nickel can be indirectly reflected, and further the nickel pollution degree of the region is indirectly reflected. According to the quantitative detection method for the nickel-IgG chelate, established by the invention, the accuracy is greatly improved, and the detection repeatability is greatly improved.

Description

technical field [0001] The invention relates to the field of detection, and more specifically relates to a nickel-IgG chelate and its preparation method and application. Background technique [0002] IgG is mainly synthesized and secreted by plasma cells in the spleen and lymph nodes, and exists in the form of monomers. In the process of individual development, the body synthesizes IgG later than IgM, and begins to synthesize it in the third month after birth, and it is close to the adult level at 3 to 5 years old. IgG is the main antibody component in serum, accounting for about 75% of total Ig in serum. IgG is the only Ig that can cross the placenta and plays an important role in natural passive immunity. In addition, IgG also has the functions of opsonizing phagocytosis, ADCC and binding SPA. Due to the above characteristics of IgG, IgG plays a major role in the immune protection of the body, and most antibacterial, antiviral, and antitoxin antibodies belong to the IgG...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/00G01N33/68G01N33/96
CPCC07K16/00G01N33/6854G01N33/96
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 上海拜豪生物科技有限公司