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High resistant starch (RS3) content product prepared through autoclaving treatment and enzymatic debranching and preparation method thereof

A technology of resistant starch and debranching, applied in the field of food processing, can solve the problems of low yield of resistant starch, complicated production process, low efficiency, etc., and achieve the effects of low cost, clean production process and simple operation.

Active Publication Date: 2015-10-28
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, currently RS 3 The production is mainly through multiple freeze-thaw cycles to increase the yield of resistant starch, the production process of this method is complicated, the efficiency is low, and the yield of resistant starch is not high

Method used

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  • High resistant starch (RS3) content product prepared through autoclaving treatment and enzymatic debranching and preparation method thereof
  • High resistant starch (RS3) content product prepared through autoclaving treatment and enzymatic debranching and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1: control sample preparation method:

[0071] The raw sweet potato starch was prepared with distilled water to obtain a sweet potato starch suspension with a concentration of 25wt%, autoclaved at 121° C. for 40 minutes, and cooled to room temperature;

[0072] The pH of the starch paste was adjusted to 2 with 2M hydrochloric acid for 1 hour, then aged at 4 °C for 24 hours, then dried at 60 °C for 48 hours, pulverized with a universal pulverizer, and passed through an 80-mesh standard sieve to obtain a control sample .

Embodiment 2

[0073] Example 2: Preparation of Resistant Starch Product A - Method 1

[0074] 1. Prepare a sweet potato starch suspension with a concentration of 25wt% from the raw sweet potato starch with distilled water, carry out autoclaving at 121°C for 40 minutes, the pressure is 0.1-0.3MPa, and cool the starch paste to 60°C;

[0075] 2. Adjust the pH of the starch paste to 6 with 2M hydrochloric acid, add 20 ASPU / g starch pullulanase, and then hydrolyze at 60°C for 12 hours;

[0076] 3. Cool the hydrolyzed starch milk to room temperature, adjust the pH of the starch paste to 2 with 2M hydrochloric acid, leave it at room temperature for 1 hour to kill the enzyme, and then store it at 4°C for 24 hours (this is the aging treatment step, the same below). ), then dried at 60° C. for 48 hours, pulverized with a universal pulverizer, and passed through an 80-mesh standard sieve to obtain a resistant starch product A.

[0077] Its process flow chart see figure 1 .

Embodiment 3

[0078] Example 3: Preparation of Resistant Starch Product A - Method 2

[0079] 1. Prepare a sweet potato starch suspension with a concentration of 20 wt % by using distilled water to prepare a sweet potato starch suspension, perform autoclaving at 121° C. for 30 minutes, at a pressure of 0.1-0.3 MPa, and cool the starch paste to 60° C.;

[0080] 2. Adjust the pH of the starch paste to 5.5 with 2M hydrochloric acid, add 22 ASPU / g starch isoamylase, and then hydrolyze at 60°C for 12 hours;

[0081] 3. Cool the hydrolyzed starch milk to room temperature, adjust the pH of the starch paste to 2 with 2M hydrochloric acid, leave it at room temperature for 1 hour to kill the enzyme, and then store it at 4°C for 24 hours (this is the aging treatment step, the same below). ), then dried at 60° C. for 48 hours, pulverized with a universal pulverizer, and passed through an 80-mesh standard sieve to obtain a resistant starch product A.

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Abstract

The invention relates to a high resistant starch (RS3) content product prepared through autoclaving treatment and enzymatic debranching and a preparation method thereof. The product is prepared by the following method that 1, native starch and water are mixed to obtain starch suspension liquid, autoclaving treatment is performed for 5-48 minutes, and cooling treatment is performed; 2, pH is regulated and enzymatic debranching treatment is performed, or after aging treatment is performed, pH is regulated and debranching treatment is performed; 3, an obtained sample is treated through enzyme deactivation, aging, drying, powder grinding and sieving, and the high resistant starch content product is prepared. The product takes the starch as the raw materials and adopts the method of combining autoclaving treatment and enzymatic debranching, the resistant starch (RS3) content in the obtained product is above 40 percent, the resistant starch content is greatly increased, the production technology is simplified, and the production cost is lowered.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for preparing highly resistant starch (RS 3 ) content of the product and its preparation method. Background technique [0002] Resistant starch (Resistant Starch, RS), also known as anti-enzymatic starch and indigestible starch, cannot be enzymatically hydrolyzed in the small intestine, but can be fermented with volatile fatty acids in the human gastrointestinal tract. This starch is more difficult to degrade than other starches, digests slowly in the body, and absorbs and enters the blood slowly. Its properties are similar to soluble fiber, and it has a certain slimming effect. In recent years, it has been favored by beauty lovers. Resistant starch can resist the decomposition of enzymes, release glucose slowly in the body, has a low insulin response, can control blood sugar balance, reduce hunger, and is especially suitable for diabetics. [0003] Resistant ...

Claims

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Application Information

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IPC IPC(8): C08B31/00
Inventor 木泰华余树玺孙红男赵仲凯
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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