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Application of hydrogen in preparation of drug for preventing limb ischemia-reperfusion injury

A technology for reperfusion injury and hydrogen, which is applied in the field of medicine to achieve the effects of obvious effects, small side effects and simple methods

Inactive Publication Date: 2015-11-04
王岱君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no research has been reported on whether intraperitoneal injection of hydrogen can prevent limb ischemia-reperfusion injury

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Effects of intraperitoneal hydrogen injection on serum MDA and SOD activities in limb ischemia-reperfusion injury.

[0015] Experimental method: Prepare rabbit limb ischemia-reperfusion injury animal model, detect serum MDA content and SOD activity, and confirm the preventive effect of intraperitoneal injection of hydrogen on limb ischemia-reperfusion injury.

[0016] Specifically, it is completed through the following steps:

[0017] 1. Hydrogen production: Use M177021 hydrogen generator (produced by Beijing Zhongxi Yuanda Technology Co., Ltd.) to generate hydrogen by electrolyzing water with a purity of 99.999%.

[0018] 2. Experimental animals: 30 healthy adult male New Zealand big-eared white rabbits, body weight (2.21±0.13) kg. After the animals were purchased, they were housed in separate cages in SPF grade animal rooms for 1 week at a room temperature of 25°C and a humidity of 40%-60%. Fasted for 12 hours before the experiment, but drank water normally.

[001...

Embodiment 2

[0030] Intraperitoneal injection of hydrogen on the effect of serum 8-iso-PGF in limb ischemia-reperfusion injury 2α levels and GSH-Px activity.

[0031] Experimental method: Prepare rabbit limb ischemia-reperfusion injury animal model, and detect serum 8-iso-PGF 2α Level and GSH-Px activity, confirming the preventive effect of intraperitoneal hydrogen injection on limb ischemia-reperfusion injury.

[0032] 1. Except for index detection, others are the same as in Example 1.

[0033] 2. Index detection: Enzyme-linked immunosorbent assay 8-iso-PGF 2α The detection kit was purchased from Cayman Chemical Company (Ann Arbor, MI). The activity of GSH-Px was determined by the dithionitrobenzoic acid method, and the detection kit was purchased from Nanjing Jiancheng Bioengineering Institute. The specific detection steps were carried out according to the kit instructions.

[0034] 3. Results and analysis: See Table 2 for the results.

[0035] Table 2 Serum 8-iso-PGF of animals in...

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Abstract

The invention discloses an application of hydrogen in preparation of a drug for preventing limb ischemia-reperfusion injury. Experimental results show that hydrogen can remarkably reduce rise of limb ischemia-reperfusion MDA (malondialdehyde) and 8-isoprostanes F2alpha (8-iso-PGF2alpha), protect the activity of SOD (superoxide dismutase) and GSH-Px (glutathione peroxidase), reduce lipid peroxidation, protect histocyte structure and function integrity, effectively protect tissue injury caused by limb ischemia-reperfusion and has great significance for clinical prevention and treatment of the limb ischemia-reperfusion injury.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of hydrogen in the preparation of medicines for preventing limb ischemia-reperfusion injury. Background technique [0002] Limb ischemia-reperfusion injury refers to the recovery of blood flow in an ischemic limb that causes tissue damage that is more severe than that caused by ischemia. Ischemia-reperfusion injury of limbs is common in orthopedic surgery, trauma surgery, vascular surgery, etc. serious complications. Although a lot of research has been done on ischemia-reperfusion injury, its mechanism is still not clear, and there is no very effective prevention and treatment method. [0003] In recent years, the biological effects of hydrogen have been extensively studied. Hydrogen is the simplest element in nature. Hydrogen is a colorless, odorless, tasteless, diatomic gas with certain reducing properties. In the past, most scholars have always...

Claims

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Application Information

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IPC IPC(8): A61K33/00A61P9/10
Inventor 王岱君田华
Owner 王岱君
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