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Response surface method for optimizing extraction process of red-fleshed pitaya peel anthocyanin

A technology of red pitaya and response surface method is applied in the field of optimizing the extraction process of anthocyanin from red pitaya peel, which can solve the problems of poor technical conditions, high production cost, environmental pollution and the like.

Inactive Publication Date: 2015-11-04
PUTIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current technical conditions for anthocyanin extraction are relatively poor, which not only causes a great waste of resources, but also causes environmental pollution, unstable processing quality, high production costs, and low economic benefits.

Method used

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  • Response surface method for optimizing extraction process of red-fleshed pitaya peel anthocyanin
  • Response surface method for optimizing extraction process of red-fleshed pitaya peel anthocyanin
  • Response surface method for optimizing extraction process of red-fleshed pitaya peel anthocyanin

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Experimental program
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Effect test

Embodiment 1

[0050] Determination of detection wavelength

[0051] Clean the dragon fruit with distilled water, then separate the pulp and peel of the dragon fruit, then dry the peel in an oven and grind the dried dragon fruit peel into powder with a traditional Chinese medicine grinder. Accurately weigh 1.0g of red dragon fruit peel powder, then add an appropriate solvent, shake it with a shaker at room temperature for an appropriate time, then put it into a centrifuge for centrifugation, and take the supernatant. The obtained supernatant was properly diluted and scanned in an ultraviolet spectrophotometer with a wavelength range of 300-800 nm, so as to determine the maximum absorption wavelength of anthocyanins. After scanning with a UV spectrophotometer, the maximum absorption peak of anthocyanins in dragon fruit peel appears at 519nm, so it is determined that the best detection wavelength for anthocyanins is 519nm.

Embodiment 2

[0053] Effects of ethanol concentration on the extraction of anthocyanins from red pitaya peel

[0054] Accurately weigh 1.0 g of dried red pitaya peel powder, add 10 mL of ethanol volume fractions of 0%, 15%, 30%, 45%, and 60% ethanol with a pH value equal to 6, and set the shaker to extract The temperature was 35°C, shaken for 30 min, and then placed in a centrifuge at 4000 r / min for 10 min, and the obtained supernatant was properly diluted, and the absorbance was measured at 519 nm to obtain figure 1 .

[0055] Figure 1 shows that different concentrations of absolute ethanol have different effects on anthocyanins from red pitaya peel. With the increase of ethanol volume fraction, the absorbance also gradually increases. When the absorbance reaches the highest peak, the corresponding ethanol volume fraction It is 30%, when it continues to increase, the absorbance not only does not increase but decreases. The main reason is that the anthocyanins in the pitaya peel are water...

Embodiment 3

[0057] Effects of extraction time on the extraction of anthocyanins from red pitaya peel

[0058] Accurately weigh 1.0 g of dried pitaya peel powder, add 10 mL of 30% ethanol solvent with a pH value of 6.0, set the extraction temperature to 35°C, and shake for 35 min, 45 min, 55 min, 65 min, and 75 min respectively , and then placed in a centrifuge at 4000 r / min for 10 min, the supernatant obtained was properly diluted and the absorbance was measured at 519 nm, as figure 2 .

[0059] Depend on figure 2 It can be obtained that with the continuous increase of time, the absorbance also increases firstly with the increase of time, and when the time is 45min, the absorbance is the maximum. When the extraction time continues to increase, the absorbance decreases with the increase of time, the main reason is that when the extraction time is too short, the reaction is not sufficient. And when the extraction time is too long, the reaction time is too long so that part of the impur...

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Abstract

The invention provides a response surface method for optimizing extraction process of red-fleshed pitaya peel anthocyanin. The peel of red-fleshed pitaya is taken as a raw material, a single-factor experimental method is utilized for exploring the effects of ethanol concentration, extraction time, extract liquid pH value and extraction temperature on the red-fleshed pitaya peel anthocyanin, the ethanol concentration, extract liquid pH value, extraction temperature and extraction time are taken as influence factors, the extracting solution absorbance of the red-fleshed pitaya peel anthocyanin is taken as a response value, a central combined experiment is utilized for mathematical modeling, the optimal ethanol concentration, extract liquid pH value, extraction temperature and extraction time of the red-fleshed pitaya peel anthocyanin are determined through the analysis of a response surface, and the extraction volume of the obtained anthocyanin is 58.9 mg / g. According to the response surface method, the benefits can be improved, the pollution of the peel to the environment can be reduced, and the value of pitaya is improved.

Description

technical field [0001] The invention relates to the technical field of peel anthocyanin extraction technology, in particular to a method for optimizing the extraction process of red pitaya peel anthocyanin by response surface method. Background technique [0002] Dragon fruit (Hylocereus undatus) is a common fruit in our daily life, also known as green dragon fruit, red dragon fruit and so on. Because dragon fruit has very high nutritional content, so dragon fruit is beneficial to human's physical and mental health. [0003] Dragon fruit not only has high nutritional content, but also contains vegetable albumin and anthocyanins which are relatively rare in other plants. There are many kinds of dragon fruit. According to the color of its peel and pulp, it can be divided into three types: red skin and white meat, red skin and red meat, and yellow skin and white meat. The plant-based albumin contained in it has a good Detoxification effect, especially for patients with heavy...

Claims

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Application Information

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IPC IPC(8): C07D311/62G06F19/00
CPCC07D311/62G16C99/00
Inventor 林国荣朱晓莹张瑛
Owner PUTIAN UNIV
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