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Method for obtaining extracellular water-soluble monascus yellow pigment through high-carbon source fermentation and application of method

A monascus yellow pigment and water-soluble technology, applied in the field of Monascus fermentation, can solve the problems of no market report, reducing the ratio of monascus red and orange pigment, and complicated product purification process, so as to eliminate adverse effects, optimize metabolism, Promising effects

Active Publication Date: 2015-11-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Monascus pigments produced by liquid fermentation of Monascus are mainly intracellular pigments. These pigments are easily soluble in polar organic solutions such as ethanol and acetone. They are all water-insoluble pigments and are subject to certain restrictions in food applications.
At the same time, liquid fermentation faces two bottlenecks: first, the high concentration of intracellular products in the fermentation process will lead to negative feedback inhibition of the products; high
At present, there is no relevant market report on the direct fermentation of a large amount of extracellular water-soluble monascus pigments, and the reduction of the ratio of monascus red and orange pigments to obtain high-purity yellow pigments.

Method used

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  • Method for obtaining extracellular water-soluble monascus yellow pigment through high-carbon source fermentation and application of method
  • Method for obtaining extracellular water-soluble monascus yellow pigment through high-carbon source fermentation and application of method
  • Method for obtaining extracellular water-soluble monascus yellow pigment through high-carbon source fermentation and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of seed liquid: inoculate the seeds of Monascus ruber (Monascus ruber) WQ15 (preservation number is CGMCC No.10910) streaked on the plate into sterilized seed medium (glucose 1g, yeast extract powder 0.15g , fish meal peptone 0.5g, KCl 0.025g, KH 2 PO 4 0.2g, FeSO 4 ·7H 2 (0.5mg, fixed to 50mL with distilled water, and the pH is natural) to cultivate and proliferate, 50ml seed culture medium inoculates 5 single bacterium colonies of cultivating 6 days, cultivates 30h at 30 ℃, 180rpm, makes the seed culture medium have mature spores, obtains seed liquid.

[0033] (2) High carbon source fermentation: the seed liquid that step (1) obtains is inoculated into the basic fermentation medium (glucose 1.25g, (NH 4 ) 2 SO 4 0.125g, KH 2 PO 4 0.125g, MgSO 4 ·7H 2 O0.0125g, KCl 0.0125g, FeSO 4 ·7H 2 O 0.25mg, ZnSO 4 ·7H 2 O 0.25mg, MnSO 4 ·H 2(0.75mg, be fixed to 25mL with distilled water, pH is natural), 30 ℃, 180rpm ferment and cultivate 3 days; Th...

Embodiment 2

[0037] (1) Preparation of seed liquid: same as step (1) of Example 1.

[0038] (2) High-carbon source fermentation: basically the same as step (2) of Example 1, the difference is only that glycerol is added in this example, replacing the glucose added in Example 1, and the amount of added glycerol is calculated per liter Add 300g of glycerol to the basic fermentation medium.

[0039] (3) conventional fermentation: with embodiment 1 step (3).

[0040] The result is as figure 1 As shown, the absorbance of the high-carbon source fermentation extracellular liquid at the characteristic wavelengths of 350, 470, and 510nm measured by an ultraviolet spectrophotometer was 68.27, 4.98, and 0.65 AU / mL, respectively, and the yellow hue of the fermented extracellular red yeast rice was 105.03, indicating that the yellow The purity of the pigment is high, and the output of extracellular yellow pigment is the same as that of conventional fermentation (10.00AU 350 / mL) 6.83 times.

Embodiment 3

[0042] (1) Preparation of seed liquid: same as step (1) of Example 1.

[0043] (2) High-carbon source fermentation: basically the same as step (2) of Example 1, the only difference is that what is added in this example is sucrose, which replaces the glucose added in Example 1, and the amount of sucrose added is calculated per liter Calculated by adding 300g sucrose to the basic fermentation medium.

[0044] (3) conventional fermentation: with embodiment 1 step (3).

[0045] The result is as figure 1 As shown, the absorbance at the characteristic wavelengths of 350, 470, and 510 nm of the high-carbon source fermentation extracellular liquid was measured by a spectrophotometer to be 108.15, 5.29, and 1.04 AU / mL, respectively, and the yellow hue of the fermented extracellular red yeast rice was 104.00, indicating that the yellow pigment High purity, extracellular yellow pigment production is conventional fermentation (10.00AU 350 / mL) 10.81 times.

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Abstract

The invention discloses a method for obtaining extracellular water-soluble monascus yellow pigment through high-carbon source fermentation and application of the method. The method comprises the following steps of inoculating a monascus strain in a basic fermentation medium for aerobic fermentation to ensure that the monascus strain is in a logarithmic growth period or stable period; then, replenishing a carbon source, and further carrying out aerobic fermentation, wherein the concentration of the carbon source in the fermentation medium is at least 60g / L; and carrying out solid-liquid separation on the fermented fermentation solution, and extracting the liquid to obtain the extracellular water-soluble monascus yellow pigment. According to the method, the metabolism of monascus is optimized by using a direct high-carbon-source fermentation method, so that the extracellular water-soluble monascus yellow pigment with a high color value and high tone is obtained, and the natural water-soluble monascus yellow pigment is produced. The current process for producing the monascus yellow pigment by using a conversion method can be replaced with the method, so that adverse effects in chemical conversion steps can be eliminated; and the obtained pure-natural monascus yellow pigment product contains no artificially-modified structural components, so that the quality requirement for food safety is met, and the application prospect is wide.

Description

technical field [0001] The invention relates to a fermentation method of Monascus, in particular to a method and application for obtaining extracellular water-soluble monascus yellow pigment through high-carbon source fermentation. Background technique [0002] Monascus pigment is a natural pigment produced by the fermentation of Monascus bacterium. It has been used as a natural food pigment in China, Korea and Japan, and is widely used in beverages, sauces, edible oils, bread, pastries and other fields. It has a history of thousands of years. . At present, the red yeast rice pigments used in commercial products are generally mixed pigments, which are mainly divided into three categories: red, orange, and yellow. There are more than 50 kinds of red yeast pigments that have been confirmed and identified, including six common intracellular pigments: 2 kinds of red pigments (Rubropunctamine, Monascorubramine), 2 kinds of orange pigments (Rubropunctatine, Monascorubrine) and 2 ...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/66
Inventor 吴振强汪梅花陈功
Owner SOUTH CHINA UNIV OF TECH
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