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Biological preparation and application of same in preparation of drugs used for preventing and controlling Ebola virus

A technology for Ebola virus and biological preparations, applied in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of complex production process, high cost and difficult problems of ZMapp

Active Publication Date: 2015-11-11
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, ZMapp is composed of three neutralizing antibodies against Zaire-type Ebola virus. Whether it can effectively protect against mutated antigens or other types of Ebola virus in possible outbreaks in the future has yet to be determined. Second, the production process of ZMapp is relatively complicated, the cost is high, and it requires relatively complete storage and transportation conditions. Therefore, how to use it for epidemic control in countries with relatively backward economies in Africa is also a difficult problem

Method used

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  • Biological preparation and application of same in preparation of drugs used for preventing and controlling Ebola virus
  • Biological preparation and application of same in preparation of drugs used for preventing and controlling Ebola virus
  • Biological preparation and application of same in preparation of drugs used for preventing and controlling Ebola virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A biological preparation for preventing and controlling multiple genotypes of Ebola virus infection, prepared by the following method:

[0070] (1) Prepare 3-hydroxy-phthalic anhydride (HP) with dimethyl sulfoxide (DMSO) to make a mother liquor with a concentration of 1M;

[0071] (2) Prepare a disodium hydrogen phosphate solution (pH8.5) with a concentration of 0.1M, add human serum albumin to make an HSA solution, and the concentration of HSA is 20mg / ml;

[0072] (3) Add the acid anhydride solution prepared in step (1) into the HSA solution to be modified, so that the final concentration of the added acid anhydride is 12mM. After mixing well, adjust the pH of the protein solution to 9.0 with 4M NaOH solution, and incubate at 25°C for 20 minutes;

[0073](4) Repeat step (3) five times in total, each time the final concentration of acid anhydride is 12mM, 24mM, 36mM, 48mM, 60mM, that is, the final concentration of acid anhydride in the solution is 60mM, incubate at 25°C...

Embodiment 2

[0077] A biological preparation for preventing and controlling multiple genotypes of Ebola virus infection, prepared by the following method:

[0078] (1) Prepare maleic anhydride (ML) with dimethyl sulfoxide (DMSO) to make a mother liquor with a concentration of 1M;

[0079] (2) Prepare a 0.1M disodium hydrogen phosphate solution (pH8.5), add bovine β-lactoglobulin (abbreviated as β-LG) to make a β-LG solution, and the concentration of β-LG is 20mg / ml;

[0080] (3) Add the acid anhydride solution prepared in step (1) to the β-LG solution to be modified, so that the final concentration of the added acid anhydride is 12mM, mix well, adjust the pH of the protein solution to 9.0 with 4M NaOH solution, and incubate at 25°C 20min;

[0081] (4) Repeat step (3) five times in total, each time the final concentration of acid anhydride is 12mM, 24mM, 36mM, 48mM, 60mM, that is, the final concentration of acid anhydride in the solution is 60mM, incubate at 25°C for 2h to complete the an...

Embodiment 3

[0085] A biological preparation for preventing and controlling multiple genotypes of Ebola virus infection, prepared by the following method:

[0086] (1) Prepare HP with dimethyl sulfoxide (DMSO) to make a mother solution with a concentration of 1M;

[0087] (2) Prepare a 0.1M disodium hydrogen phosphate solution (pH 8.5), add ovalbumin (ovalbumin, abbreviated as OVA) to make an OVA solution, and the OVA concentration is 20mg / ml;

[0088] (3) Add the acid anhydride solution prepared in step (1) to the OVA solution to be modified, so that the final concentration of the added acid anhydride is 12mM. After mixing well, adjust the pH of the protein solution to 9.0 with 4M NaOH solution, and incubate at 25°C for 20 minutes;

[0089] (4) Repeat step (3) five times in total, each time the final concentration of acid anhydride is 12mM, 24mM, 36mM, 48mM, 60mM, that is, the final concentration of acid anhydride in the solution is 60mM, incubate at 25°C for 2h to complete the anhydride ...

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Abstract

The invention discloses a biological preparation used for preventing and controlling multiple genotypes of Ebola virus infection. A preparation method for the biological preparation comprises the following steps: preparing a mother liquor with a concentration of 1 M from acid anhydride and dimethyl sulfoxide; preparing a disodium hydrogen phosphate solution with a concentration of 0.1 M and applying the disodium hydrogen phosphate solution in preparation of a protein solution with a protein concentration of 20 mg / ml; adding the acid anhydride solution into the to-be-modified protein solution, wherein the concentration of added acid anhydride is allowed to be 12 mM, carrying out full and uniform mixing, adjusting the pH value of the protein solution to 9.0 and carrying out incubation at 25 DEG C for 20 min; repeating mixing of the acid anhydride and the protein solution until eventually, the final concentration of the acid anhydride in the protein solution is 60 mM and the pH value of the solution is 9.0, and carrying out incubation at 25 DEG C for 2 h to accomplish acid anhydride reaction; and filling a dialysis bag with the acid anhydridated protein solution, putting the dialysis bag in a phosphatic buffer with a pH value of 7.4 for dialysis for 48 h, filtering the acid anhydridated protein solution with a microfiltration membrane with a size of 0.45 [mu]m and carrying out preservation at 4 DEG C.

Description

technical field [0001] The invention relates to a novel antiviral biological agent in the field of medical technology, in particular to a biological agent for preventing and controlling Ebola virus infection. Background technique [0002] Ebola virus disease (EVD) is a viral infectious disease caused by Ebola virus (EBOV) infection and transmitted through body fluids or close contact. The natural host of Ebola virus is fruit bats, which are mainly pathogenic to primates including humans. Since the discovery of Ebola virus in the 1970s, the virus infection has been sporadic in Central Africa Local outbreak, but the Ebola outbreak in West Africa that spread from 2014 to this year far exceeds any outbreak in history. According to WHO statistics, as of April 8, 2015, the total number of infected people was 25,550, of which more than 10,587 died . The incubation period of the disease is 2 to 21 days. The early symptoms are fever, gastrointestinal discomfort, muscle pain, etc. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/38A61P31/14
Inventor 姜世勃陆路李浩洋于飞王茜杨霞
Owner FUDAN UNIV
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