siRNA capable of inhibiting MAT2A genetic expression and application of siRNA

A gene expression and gene technology, applied to the siRNA that inhibits the expression of the MAT2A gene and its application field, to achieve the effect of inhibiting angiogenesis in liver cancer, high theoretical and practical value
CN105039342AActive Publication Date: 2015-11-11李家平 +3
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
李家平
Publication Date
2015-11-11

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Abstract

The invention discloses siRNA capable of inhibiting MAT2A genetic expression and application of the siRNA. The positive-sense strand of siRNA MAT2A-1 capable of inhibiting the MAT2A genetic expression is shown as SEQ ID NO:2, and the antisense strand of the siRNA MAT2A-1 is shown as SEQ ID NO:3. The positive-sense strand of siRNA MAT2A-2 capable of inhibiting the MAT2A genetic expression is shown as SEQ ID NO:4, and the antisense strand of the siRNA MAT2A-2 is shown as SEQ ID NO:5. Lentivirus vector transfection liver cancer cells of lentivirus expression vector combination overexpression MAT1A genes built according to the siRNA inhibiting the MAT2A genetic expression successfully inhibit hepatocellular carcinoma angiogenesis, migration, invasion and lung metastasis, the nucleotide sequence of the MAT1A genes is shown as the 256th-144th bases of SEQ ID NO.1. The siRNA can be further applied to preparing medicine treating liver cancer diseases and has high theoretical and application value.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to siRNA capable of inhibiting MAT2A gene expression and application thereof. Background technique

[0002] Hepatocellular carcinoma (Hepatocellular carcinoma, HCC) is one of the most common malignant tumors, ranking second in the incidence of cancer in the country, and the number of deaths due to liver cancer is about 350,000 every year. Currently, surgical resection and liver transplantation are the most effective treatments for liver cancer. However, due to the low rate of early diagnosis and the shortage of liver transplant donors, most patients lost the chance of radical surgery, and the 5-year survival rate of patients was only 10-20%. Transarterial chemoembolization (TACE) is the main treatment for unresectable liver cancer, which can promote tumor ischemic necrosis and inhibit its growth, but embolization is difficult to complete, and postoperative recurrence and meta...

Claims

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