Application of RBP4 as colorectal cancer blood serum marker and diagnostic kit

A diagnostic kit, colorectal cancer technology, applied in the biological field, can solve the problem of not finding tumor markers, etc., and achieve the effect of assisting early diagnosis of colorectal cancer, high specificity and sensitivity

Inactive Publication Date: 2015-11-11
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the occurrence and development of colorectal cancer is the result of multiple gene damage, and no i...

Method used

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  • Application of RBP4 as colorectal cancer blood serum marker and diagnostic kit
  • Application of RBP4 as colorectal cancer blood serum marker and diagnostic kit
  • Application of RBP4 as colorectal cancer blood serum marker and diagnostic kit

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Experimental program
Comparison scheme
Effect test

Embodiment

[0020] 1. Collection and preparation of serum samples:

[0021] Whole blood samples from patients with colorectal cancer and normal controls were collected, placed at room temperature for 2 hours and centrifuged at 3000g for 5 minutes, and the supernatant was taken to obtain serum. The obtained serum can be used immediately for detection, or stored at -80°C after aliquoting.

[0022] 2. Configuration methods of various buffer solutions and reagents for ELISA

[0023] (1) Coating buffer: PH9.6, 0.05M carbonate buffer

[0024]

[0025] (2) Sample diluent: PH7.4, 0.01mol / L PBS buffer

[0026]

[0027] (3) Washing buffer: PH7.4, 0.01M PBST buffer (containing 0.05% Tween-20)

[0028]

[0029] (4) Blocking solution: pH 7.4 PBS solution containing 0.5% BSA.

[0030]

[0031] (5) Substrate chromogenic solution:

[0032] Chromogenic Solution A

[0033]

[0034] Chromogenic solution B: (prepared and used now)

[0035]

[0036] (6) Stop buffer: 2MH 2 SO 4

[0...

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Abstract

The invention discloses application of an RBP4 as a colorectal cancer blood serum marker and a diagnostic kit, and belongs to the technical field of biology. On one hand, the invention provides the technical scheme of the application of the RBP4 as the colorectal cancer blood serum marker, and on the other hand, the invention provides the technical scheme of the colorectal cancer diagnostic kit. The advantages that the blood serum biological marker has the specificity of 81.7% and the sensibility of 74.9% and has high specificity and sensibility, a flexible, reliable and operable method is provided, the antigen level of the RBP4 in human blood serum is quantitatively measured and the RBP4 can easily assist in colorectal cancer early diagnosis are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of RBP4 as a serum marker for colorectal cancer and a diagnostic kit. Background technique [0002] Colorectal cancer is one of the most common malignant tumors. In the world, the mortality rate of colorectal cancer ranks among the top three. In China, the incidence of colorectal cancer is on the rise, and the mortality rate ranks fifth. After active surgical treatment and radiotherapy and chemotherapy, the 5-year overall survival rate of colorectal cancer patients is about 66%. Data show that if colorectal patients are effectively diagnosed and treated at stage I or even earlier, the survival rate can increase to more than 90%. However, the current early diagnosis rate of colorectal cancer is only about 37%. Once the treatment is discovered at stage III or IV, the survival rate will be reduced to 10%-30%. It can be seen that it is very important to impr...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/6893G01N33/57419G01N2333/4703
Inventor 胡晓彤何超费伟强
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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